Xu Chu, Yang Yufei, Shao Zhurui, Ren Ruizi, Zhang Yiwen, Jin Yipeng, Shi Hao
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, China.
China Agricultural University Veterinary Teaching Hospital, Beijing, China.
Am J Vet Res. 2024 Feb 5:1-12. doi: 10.2460/ajvr.23.09.0214.
To identify metabolites and metabolic pathways affected in dogs with struvite and calcium oxalate urolithiasis compared to healthy dogs. To explore the candidate urinary biomarkers to distinguish dogs with struvite and calcium oxalate urolithiasis.
13 dogs with calcium oxalate urolithiasis, 7 dogs with struvite urolithiasis, and 13 healthy dogs were recruited between September 2021 and January 2023.
Metabolomic profiles were analyzed from urine samples using UPLC-MS MS. According to the variable importance in the projection (> 1) and correlation coefficient (P < .05) obtained by orthogonal partial least squares discriminant analysis, the differential metabolites were screened. The Kyoto Encyclopedia of Genes and Genomes database was used to identify the metabolic pathways involved.
Compared to healthy dogs, those with calcium oxalate urolithiasis exhibited distinct metabolites primarily associated with phenylalanine metabolism, nicotinic acid, and nicotinamide metabolic pathways. Conversely, dogs with struvite urolithiasis demonstrated variations in metabolites mainly linked to tryptophan metabolism and glycerophospholipid metabolic pathways. Between calcium oxalate and struvite groups, pyocyanin, glycylprolylarginine, traumatin, cysteinyl-leucine, and 8-hydroxydodecylcarnitine are candidate urinary biomarkers.
Our findings provide an in-depth analysis of metabolic perturbations associated with calcium oxalate and struvite urolithiasis in dogs. We also identified candidate urinary biomarkers distinguishing between dogs with calcium oxalate and struvite urolithiasis, which can be subsequently validated to assist in stone diagnosis and guide treatment choices.
与健康犬相比，确定患有鸟粪石和草酸钙尿石症的犬体内受影响的代谢产物和代谢途径。探索用于区分患有鸟粪石和草酸钙尿石症的犬的候选尿液生物标志物。
2021年9月至2023年1月期间招募了13只患有草酸钙尿石症的犬、7只患有鸟粪石尿石症的犬和13只健康犬。
使用超高效液相色谱-质谱联用仪对尿液样本进行代谢组学分析。根据通过正交偏最小二乘法判别分析获得的投影变量重要性（>1）和相关系数（P<0.05）筛选差异代谢产物。使用京都基因与基因组百科全书数据库确定涉及的代谢途径。
与健康犬相比，患有草酸钙尿石症的犬表现出明显的代谢产物，主要与苯丙氨酸代谢、烟酸和烟酰胺代谢途径相关。相反，患有鸟粪石尿石症的犬的代谢产物变化主要与色氨酸代谢和甘油磷脂代谢途径有关。在草酸钙组和鸟粪石组之间，绿脓菌素、甘氨酰脯氨酰精氨酸、创伤激素、半胱氨酰亮氨酸和8-羟基十二烷肉碱是候选尿液生物标志物。
我们的研究结果对犬草酸钙和鸟粪石尿石症相关的代谢紊乱进行了深入分析。我们还确定了区分患有草酸钙和鸟粪石尿石症的犬的候选尿液生物标志物，随后可对其进行验证，以协助结石诊断并指导治疗选择。
