Department of Biochemistry and Molecular Medicine, The George Washington University, Washington, DC, United States.
Department of Biochemistry and Molecular Medicine, The George Washington University, Washington, DC, United States.
Methods Cell Biol. 2024;181:59-72. doi: 10.1016/bs.mcb.2022.09.005. Epub 2022 Oct 29.
Cellular senescence, whereby cells cease to proliferate, is known to contribute to the aging process and age-related pathologies. It is elicited either by cell-intrinsic mechanisms such as progressive telomere shortening or due to the extrinsic stress-related factors, which via p53-p21 and p16-pRB tumor suppressor pathways signal cells to cease proliferation. A proper identification and characterization of senescent cells is necessary to understand the process of aging, age-related pathologies, and the development of therapeutics to treat age-related dysfunctions. The landmark discovery of Senescence-Associated-Beta-Galactosidase (SA-β-Gal) marker, and a simple colorimetric method to detect SA-β-Gal greatly facilitated identification of the senescent cells in human and rodent cells pertaining to age-related diseases (Dimri et al., 1995). Despite the availability of additional senescence biomarkers, the SA-β-Gal marker and histochemical detection method remain the most widely used tool to identify senescent cells in vitro and in vivo. Here, we revisit the original colorimetric method to detect senescent cells that was first published in 1995 (Dimri et al., 1995).
细胞衰老，即细胞停止增殖，已知会导致衰老过程和与年龄相关的病理发生。它是由细胞内在机制（如端粒逐渐缩短）引起的，也可能是由于外在的应激相关因素引起的，这些因素通过 p53-p21 和 p16-pRB 肿瘤抑制途径信号通知细胞停止增殖。正确识别和描述衰老细胞对于理解衰老过程、与年龄相关的病理以及开发治疗与年龄相关的功能障碍的疗法是必要的。β-半乳糖苷酶（SA-β-Gal）标志物的发现以及检测 SA-β-Gal 的简单比色法极大地促进了与年龄相关疾病的人类和啮齿动物细胞中衰老细胞的鉴定（Dimri 等人，1995 年）。尽管有其他衰老生物标志物可用，但 SA-β-Gal 标志物和组织化学检测方法仍然是体外和体内鉴定衰老细胞最广泛使用的工具。在这里，我们重新审视了 1995 年首次发表的检测衰老细胞的比色法（Dimri 等人，1995 年）。
