Murali Preethi, Kavitha B, Narasimhan Malathi
Department of Oral and Maxillofacial Pathology and Oral Microbiology, Meenakshi Ammal Dental College and Hospital, MAHER, Chennai, Tamil Nadu, India.
Department of Oral and Maxillofacial Pathology, Sri Ramachandra Dental College and Hospitals, SRIHER, Chennai, Tamil Nadu, India.
J Oral Maxillofac Pathol. 2023 Oct-Dec;27(4):623-628. doi: 10.4103/jomfp.jomfp_311_23. Epub 2023 Dec 20.
Oral cancer is one of the top three types of cancer and is of significant public health importance in India. A common post-translational modification in cells is ubiquitination/deubiquitination, and its dysregulation is closely associated with the development of cancer. Studies on the role of ubiquitination in oral squamous cell carcinoma (OSCC) are lacking. Increased expression of has been observed in various types of cancer, and this study aimed to check the gene expression of in OSCC patients. In this study, we analyzed the expression of ubiquitin-specific proteases (USPs) 36 in OSCC.
A total of 15 OSCC patients at different stages of tumor differentiation and age- and sex-matched controls were recruited for the study. The patients were categorized based on their differentiation patterns. RNA was extracted from the tissues, and gene expression was checked in these samples using a quantitative real-time PCR technique.
Our study showed increased expression of gene in OSCC patients. The mRNA was 231.8 ± 137.94 folds higher in well-differentiated squamous cell carcinoma patients, 38.18 ± 3.77 folds higher in moderately differentiated squamous cell carcinoma patients, and 25.49 ± 7.30 folds higher in poorly differentiated squamous cell carcinoma patients compared to control tissues.
Our study reports, for the first time, an increased gene expression of in OSCC tissues.
口腔癌是三大癌症类型之一，在印度具有重大的公共卫生意义。细胞中一种常见的翻译后修饰是泛素化/去泛素化，其失调与癌症的发展密切相关。关于泛素化在口腔鳞状细胞癌（OSCC）中的作用的研究尚缺。在各种类型的癌症中均观察到 的表达增加，本研究旨在检测OSCC患者中 的基因表达。在本研究中，我们分析了泛素特异性蛋白酶（USP）36在OSCC中的表达。
本研究共招募了15例处于不同肿瘤分化阶段的OSCC患者以及年龄和性别匹配的对照组。患者根据其分化模式进行分类。从组织中提取RNA，并使用定量实时PCR技术检测这些样本中的 基因表达。
我们的研究显示OSCC患者中 基因表达增加。与对照组织相比，高分化鳞状细胞癌患者的 mRNA高231.8±137.94倍，中分化鳞状细胞癌患者高38.18±3.77倍，低分化鳞状细胞癌患者高25.49±7.30倍。
我们的研究首次报道了OSCC组织中 基因表达增加。
