Liu Jiahao, Lu Jiahao, Ji Wenliang, Lu Guangwen, Wang Jiao, Ye Tingyan, Jiang Yisha, Zheng Juanjuan, Yu Ping, Liu Nannan, Jiang Yanan, Mao Lanqun
Key Laboratory of Carbon Materials of Zhejiang Province, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325027, China.
College of Chemistry, Beijing Normal University, Beijing 100875, China.
Anal Chem. 2024 Feb 13;96(6):2651-2657. doi: 10.1021/acs.analchem.3c05366. Epub 2024 Feb 2.
In vivo sensing of the dynamics of ions with high selectivity is essential for gaining molecular insights into numerous physiological and pathological processes. In this work, we report an ion-selective micropipette sensor (ISMS) through the integration of functional crown ether-encapsulated metal-organic frameworks (MOFs) synthesized in situ within the micropipette tip. The ISMS features distinctive sodium ion (Na) conduction and high selectivity toward Na sensing. The selectivity is attributed to the synergistic effects of subnanoconfined space and the specific coordination of 18-crown-6 toward potassium ions (K), which largely increase the steric hindrance and transport resistance for K to pass through the ISMS. Furthermore, the ISMS exhibits high stability and sensitivity, facilitating real-time monitoring of Na dynamics in the living rat brain during spreading of the depression events process. In light of the diversity of crown ethers and MOFs, we believe this study paves the way for a nanofluidic platform for in vivo sensing and neuromorphic electrochemical sensing.
对离子动力学进行高选择性的体内传感对于深入了解众多生理和病理过程的分子机制至关重要。在这项工作中，我们报告了一种离子选择性微吸管传感器（ISMS），它是通过将在微吸管尖端原位合成的功能性冠醚封装金属有机框架（MOF）集成而成。该ISMS具有独特的钠离子（Na）传导特性和对Na传感的高选择性。这种选择性归因于亚纳米受限空间的协同效应以及18-冠-6对钾离子（K）的特异性配位，这大大增加了K通过ISMS的空间位阻和传输阻力。此外，ISMS表现出高稳定性和灵敏度，便于在去极化事件传播过程中实时监测活大鼠脑中的Na动态。鉴于冠醚和MOF的多样性，我们相信这项研究为体内传感和神经形态电化学传感的纳米流体平台铺平了道路。
