Medical Physiology Department, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt.
Medical Physiology Department, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt.
Int Immunopharmacol. 2024 Mar 10;129:111627. doi: 10.1016/j.intimp.2024.111627. Epub 2024 Feb 3.
The current research aims to study the therapeutic efficacy of alpha-lipoic acid (α-LA) and caffeine-loaded chitosan nanoparticles (Caf-CNs) against cardiovascular complications induced by obesity. Rats were divided randomly into: control, high fat diet (HFD) induced obesity rat model, obese rats treated with α-LA and/or Caf-CNs. Triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), Interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) as well as activities of lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) significantly increased in the serum of obese rats. In addition, plasma atherogenic index, atherogenic coefficient and Castelli's risk indices I and II showed a significant increase. Additionally, levels of malondialdehyde (MDA) and nitric oxide (NO) and activity of monoamine oxidase (MAO) were significantly elevated in heart tissues of obese rats. However, cardiac Na/K-ATPase and acetylcholinesterase (AchE) activities and reduced glutathione (GSH), serotonin (5-HT), norepinephrine (NE) and dopamine (DA) as well as serum high-density lipoprotein cholesterol (HDL-C) were significantly reduced in obese rats. Treatment with α-LA and/or Caf-CNs ameliorated almost all the biochemical and histopathological alterations caused by obesity. In conclusion, the present data revealed that α-LA and/or Caf-CNs may be an effective therapeutic approach against cardiac complications caused by obesity through their antilipemic, anti-atherogenic, antioxidant, and anti-inflammatory activities.
本研究旨在探讨硫辛酸（α-LA）和载咖啡因壳聚糖纳米粒（Caf-CNs）治疗肥胖诱导的心血管并发症的疗效。大鼠随机分为：对照组、高脂肪饮食（HFD）诱导肥胖大鼠模型组、肥胖大鼠给予α-LA 和/或 Caf-CNs 治疗组。肥胖大鼠血清中甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、极低密度脂蛋白胆固醇（VLDL-C）、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）以及乳酸脱氢酶（LDH）和肌酸激酶同工酶-MB（CK-MB）活性显著升高。此外，血浆致动脉粥样硬化指数、致动脉粥样硬化系数和卡斯特利风险指数 I 和 II 显著增加。此外，肥胖大鼠心脏组织中丙二醛（MDA）和一氧化氮（NO）水平以及单胺氧化酶（MAO）活性显著升高。然而，肥胖大鼠心脏组织中 Na/K-ATP 酶和乙酰胆碱酯酶（AchE）活性以及还原型谷胱甘肽（GSH）、血清 5-羟色胺（5-HT）、去甲肾上腺素（NE）和多巴胺（DA）水平以及高密度脂蛋白胆固醇（HDL-C）显著降低。α-LA 和/或 Caf-CNs 治疗改善了肥胖引起的几乎所有生化和组织病理学改变。综上所述，本研究数据表明，α-LA 和/或 Caf-CNs 可能通过其调脂、抗动脉粥样硬化、抗氧化和抗炎作用成为治疗肥胖引起的心脏并发症的有效治疗方法。
