Peterson D L, Shires T K, Krieter P A
J Appl Biochem. 1985 Dec;7(6):396-407.
A modified peptide mapping technique is described that allows the survey of the primary structure of proteolytic fragments of particular newly translated proteins in reticulocyte lysate. The technique is demonstrated with rat liver cytochrome b5.
本文描述了一种改进的肽图谱技术,该技术可用于研究网织红细胞裂解液中特定新翻译蛋白质的蛋白水解片段的一级结构。以大鼠肝细胞色素b5为例展示了该技术。