School of Biotechnology, Shri Mata Vaishno Devi University, Katra, J&K, 182320, India.
Department of Obstetrics and Gynecology, Government Medical College, Jammu, J&K, 180001, India.
Microbes Infect. 2024 May-Jun;26(4):105308. doi: 10.1016/j.micinf.2024.105308. Epub 2024 Feb 2.
The causes of idiopathic infertility are still not known; however, it may be associated with microbial etiologies. The present study examines the vaginal microbiota of infertile as well as fertile women longitudinally.
The study was presented and accepted by the Institutional Ethical Committee of Shri Mata Vaishno Devi University, Katra, Jammu and Kashmir (India). An observational, prospective, multicenteric investigation was conducted at the Department of Obstetrics and Gynecology, Government Medical College Jammu, and its affiliated hospitals in Jammu and Kashmir (India). In order to examine the microbial composition, a cohort of 80 female individuals were involved in the screening process. The investigation involved sequencing of the V3-V4 region of 16S rRNA gene, which was subsequently analyzed using the Mothur pipeline.
The study revealed that the vaginal microbiota of infertile women differed from that of healthy women who had previously given birth without any complications. Both populations have variations in their alpha as well as beta diversity and taxonomical composition. The microbial profiles in the cases of infertility are characterized by elevated levels of Gardnerella, Prevotella, Atopobium, and Enterococcus whereas a higher level of Lactobacillus iners was observed in case of fertile women.
In conclusion, it can be inferred that the composition of the vaginal microbiome potentially exerts a significant influence on females afflicted with idiopathic infertility.
特发性不孕的病因尚不清楚；然而，它可能与微生物病因有关。本研究对不孕和有生育能力的女性的阴道微生物群进行了纵向研究。
该研究由印度查谟和克什米尔邦 Shri Mata Vaishno Devi 大学的机构伦理委员会提出并接受。在查谟的政府医学院妇产科及其附属医院进行了一项观察性、前瞻性、多中心的调查。为了检查微生物的组成，对 80 名女性个体进行了筛查过程。该调查涉及 16S rRNA 基因 V3-V4 区域的测序，随后使用 Mothur 管道进行分析。
该研究表明，不孕女性的阴道微生物群与以前没有任何并发症生育的健康女性不同。这两个群体在 alpha 和 beta 多样性以及分类组成方面都存在差异。不孕病例中的微生物谱表现为 Gardnerella、Prevotella、Atopobium 和 Enterococcus 的水平升高，而在有生育能力的女性中则观察到更高水平的 Lactobacillus iners。
总之，可以推断阴道微生物组的组成可能对患有特发性不孕的女性产生重大影响。
