通过等电沉淀法制备的大鼠肝微粒体对酶诱导的检测。

The detection of enzyme induction by rat liver microsomes prepared by isoelectric precipitation.

作者信息

Parkinson A, Safe S

出版信息

J Pharm Pharmacol. 1979 Jul;31(7):444-7. doi: 10.1111/j.2042-7158.1979.tb13550.x.

DOI:10.1111/j.2042-7158.1979.tb13550.x

Abstract

A comparison was made of various indices of the hepatic drug-metabolizing apparatus associated with the postmitochondrial superntant, microsomes harvested by differential centrifugation, and microsomes harvested by isoelectric precipitation from control, phenobarbitone-pretreated and 3-methylcholanthrene-pretreated rats. The metabolic capabilities and distinctions between control and induced rats for each of the three liver preparations compared favourably as determined by the concentration of cytochrome b5 and P-450 and by the activity of NADPH-cytochrome c reductase, 4-dimethylaminoantipyrine N-demethylase and aryl hydrocarbon (benzo[a]pyrene) hydroxylase. The results suggest that the relatively simple isoelectric precipitation technique is a useful alternative to the conventional method of differential centrifugation for the preparation of hepatic microsomes.

摘要

对来自对照、苯巴比妥预处理和3-甲基胆蒽预处理大鼠的线粒体后上清液、通过差速离心收获的微粒体以及通过等电沉淀收获的微粒体相关的肝脏药物代谢装置的各种指标进行了比较。通过细胞色素b5和P-450的浓度以及NADPH-细胞色素c还原酶、4-二甲基氨基安替比林N-脱甲基酶和芳烃（苯并[a]芘）羟化酶的活性来确定，三种肝脏制剂中对照大鼠和诱导大鼠之间的代谢能力和差异表现良好。结果表明，相对简单的等电沉淀技术是一种用于制备肝脏微粒体的常规差速离心方法的有用替代方法。