一种用于苯二氮䓬类药物的放射受体测定法。
A radioreceptor assay for benzodiazepines.
作者信息
Hunt P, Husson J M, Raynaud J P
出版信息
J Pharm Pharmacol. 1979 Jul;31(7):448-51. doi: 10.1111/j.2042-7158.1979.tb13551.x.
A simple, rapid and sensitive radioreceptor assay for determining benzodiazepines in serum is based on the displacement by the drug of specific [3H]diazepam binding to a membrane fraction from rat brain. The limit of detection of the more active benzodiazepines is about 0.5 ng. Diazepam, nitrazepam, clobazam and HR 458 have been assayed in human serum after a single oral clinical dose. The results can be used for determining pharmacokinetic parameters. The technique measures not only the parent benzodiazepine but also clinically active metabolites.
一种用于测定血清中苯二氮䓬类药物的简单、快速且灵敏的放射受体分析法，是基于药物取代特异性[³H]地西泮与大鼠脑细胞膜部分的结合。活性较高的苯二氮䓬类药物的检测限约为0.5纳克。在单次口服临床剂量后，已对人血清中的地西泮、硝西泮、氯巴占和HR 458进行了分析。这些结果可用于确定药代动力学参数。该技术不仅能测定母体苯二氮䓬类药物，还能测定具有临床活性的代谢产物。
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