Bugrov Alexander, Karamysheva Tatyana, Buleu Olesya
Novosibirsk State University, Pirogova Str. 2, Novosibirsk 630090, Russia Novosibirsk State University Novosibirsk Russia.
Institute of Systematics and Ecology of Animals, Russian Academy of Sciences, Siberian Branch, Frunze str. 11, 630091, Novosibirsk, Russia Institute of Systematics and Ecology of Animals, Russian Academy of Sciences Novosibirsk Russia.
Comp Cytogenet. 2024 Jan 25;18:15-26. doi: 10.3897/compcytogen.18.115784. eCollection 2024.
This study provides data on chromosome number (2n♂♀=26), sex determination mechanism (XY♂/XX♀), C-banding pattern, distribution of clusters of telomeric TTAGG repeats and 18S ribosomal DNA in the karyotype of the stonefly (McLachlan, 1872). For the first time in the history of stoneflies cytogenetics, we provide photos of the chromosomes of the Plecoptera insects. The karyotype of males and females of consists of 12 pairs of autosomes. Three pairs of large autosomes and four pairs of medium-sized autosomes are subacrocentric. The remaining pairs of autosomes are small, with unclear morphology. Pericentromeric C-bands were revealed in all autosomes. The sex chromosomes are also subacrocentric. The short arms of X and Y chromosomes are entirely heterochromatic and are rich in ribosomal DNA sequences. In the X chromosome this arm is larger than in the Y chromosome. It is likely that this arm associated with the nucleolar organizer (NOR). Telomeric DNA (TTAGG) repeats were detected in the terminal regions of all chromosomes.
本研究提供了关于石蝇（麦克拉克伦，1872年）核型中的染色体数目（2n♂♀=26）、性别决定机制（XY♂/XX♀）、C带模式、端粒TTAGG重复序列簇的分布以及18S核糖体DNA的相关数据。在石蝇细胞遗传学史上，我们首次提供了襀翅目昆虫染色体的照片。 的雄性和雌性核型均由12对常染色体组成。三对大的常染色体和四对中等大小的常染色体为亚近端着丝粒染色体。其余的常染色体对较小，形态不清晰。在所有常染色体上均发现了着丝粒周围C带。性染色体也是亚近端着丝粒染色体。X和Y染色体的短臂完全是异染色质，富含核糖体DNA序列。在X染色体上，这条臂比Y染色体上的大。这条臂可能与核仁组织区（NOR）相关。在所有染色体的末端区域均检测到端粒DNA（TTAGG）重复序列。
