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电磁场对可可发酵中微生物生长的影响:一种使用既定生长模型的对照实验方法。

Electromagnetic fields effects on microbial growth in cocoa fermentation: A controlled experimental approach using established growth models.

作者信息

Guzmán-Armenteros Tania María, Villacís-Chiriboga José, Guerra Luis Santiago, Ruales Jenny

机构信息

Departamento de Ciencia de Alimentos y Biotecnología (DECAB), Escuela Politécnica Nacional (EPN), Quito, Ecuador.

Escuela Superior Politécnica del Litoral, Facultad de Ingeniería Mecánica y Ciencias de la Producción, carrera de Ingeniería en Alimentos, Guayaquil, Ecuador.

出版信息

Heliyon. 2024 Jan 21;10(3):e24927. doi: 10.1016/j.heliyon.2024.e24927. eCollection 2024 Feb 15.


DOI:10.1016/j.heliyon.2024.e24927
PMID:38317962
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10839996/
Abstract

Understanding the effects of electromagnetic fields is crucial in the fermentation of cocoa beans, since through precise control of fermentation conditions the sensory and nutritional properties of cocoa beans could be improved. This study aimed to evaluate the effect of oscillating magnetic fields (OMF) on the kinetic growth of the core microbial communities of the Collections Castro Naranjal (CCN 51) cocoa bean. The data was obtained by three different models: Gompertz, Baranyi, and Logistic. The cocoa beans were subjected to different OMF strengths ranging from 0 mT to 80 mT for 1 h using the Helmholtz coil electromagnetic device. The viable microbial populations of lactic acid bacteria (LAB), acetic acid bacteria (AAB), and yeast (Y) were quantified using the colony-forming unit (CFU) counting method. The logistic model appropriately described the growth of LAB and Y under magnetic field exposure. Whereas the Baranyi model was suitable for describing AAB growth. The microbial populations in cocoa beans exposed to magnetic fields showed lower (maximum specific growth rate (μmax), values than untreated controls, with AAB exhibiting the highest average growth rate value at 5 mT and Y having the lowest average maximum growth rate value at 80 mT. The lower maximum specific growth rates and longer lag phases when exposed to magnetic fields compared to controls demonstrate the influence of magnetic fields on microbial growth kinetics.

摘要

了解电磁场的影响对于可可豆的发酵至关重要,因为通过精确控制发酵条件,可以改善可可豆的感官和营养特性。本研究旨在评估振荡磁场(OMF)对卡斯特罗·纳兰哈尔(CCN 51)可可豆核心微生物群落动力学生长的影响。数据通过三种不同模型获得:Gompertz模型、Baranyi模型和Logistic模型。使用亥姆霍兹线圈电磁装置,将可可豆置于0 mT至80 mT的不同OMF强度下处理1小时。采用菌落形成单位(CFU)计数法对乳酸菌(LAB)、醋酸菌(AAB)和酵母菌(Y)的活菌数量进行定量。Logistic模型适当地描述了磁场暴露下LAB和Y的生长情况。而Baranyi模型适用于描述AAB的生长。暴露于磁场的可可豆中的微生物数量显示出比未处理对照更低的(最大比生长速率(μmax))值,其中AAB在5 mT时表现出最高的平均生长速率值,而Y在80 mT时具有最低的平均最大生长速率值。与对照相比,暴露于磁场时较低 的最大比生长速率和较长的延迟期证明了磁场对微生物生长动力学的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/f9a8974cf67c/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/080447c59428/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/b0ed97598784/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/64c8efab8849/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/3fa8c9b73bb4/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/6e737d0b7b57/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/49ec21200538/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/d38b06c524f4/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/f9a8974cf67c/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/080447c59428/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/b0ed97598784/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/64c8efab8849/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/3fa8c9b73bb4/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/6e737d0b7b57/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/49ec21200538/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/d38b06c524f4/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/10839996/f9a8974cf67c/gr8.jpg

相似文献

[1]
Electromagnetic fields effects on microbial growth in cocoa fermentation: A controlled experimental approach using established growth models.

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[2]
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[3]
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[4]
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[5]
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[6]
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[7]
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[8]
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[9]
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[10]
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引用本文的文献

[1]
Pulsed electric field at resonance frequency combat Klebsiella pneumonia biofilms.

Appl Microbiol Biotechnol. 2024-11-5

[2]
A Review of Electromagnetic Fields in Cellular Interactions and Cacao Bean Fermentation.

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本文引用的文献

[1]
Optimization of cacao beans fermentation by native species and electromagnetic fields.

Heliyon. 2023-4-6

[2]
Modeling the Growth of Six Strains in Smoked Salmon Pâté.

Foods. 2023-3-7

[3]
Fine Cocoa Fermentation with Selected Lactic Acid Bacteria: Fermentation Performance and Impact on Chocolate Composition and Sensory Properties.

Foods. 2023-1-11

[4]
Biological effects of rotating magnetic field: A review from 1969 to 2021.

Prog Biophys Mol Biol. 2023-3

[5]
The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.

Sci Rep. 2022-5-25

[6]
Exploring cocoa bean fermentation mechanisms by kinetic modelling.

R Soc Open Sci. 2022-2-16

[7]
The Impact of Intraspecies Variability on Growth Rate and Cellular Metabolic Activity of Bacteria Exposed to Rotating Magnetic Field.

Pathogens. 2021-11-4

[8]
Functional yeast starter cultures for cocoa fermentation.

J Appl Microbiol. 2022-7

[9]
Dynamics of cocoa fermentation and its effect on quality.

Sci Rep. 2021-8-18

[10]
Microrisk Lab: An Online Freeware for Predictive Microbiology.

Foodborne Pathog Dis. 2021-8

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