利用荧光寿命成像显微镜在体评估肿瘤中的氧含量。

Oxygen Assessment in Tumors In Vivo Using Phosphorescence Lifetime Imaging Microscopy.

机构信息

Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod, Russia.

Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia.

出版信息

Methods Mol Biol. 2024;2755:91-105. doi: 10.1007/978-1-0716-3633-6_6.

DOI:10.1007/978-1-0716-3633-6_6

PMID:38319571

Abstract

The oxygen level in a tumor is a crucial factor for its development and response to therapies. Phosphorescence lifetime imaging (PLIM) with the use of phosphorescent oxygen probes is a highly sensitive, noninvasive optical technique for the assessment of molecular oxygen in living cells and tissues. Here, we present a protocol for microscopic mapping of oxygen distribution in a mouse tumor model in vivo. We demonstrate that PLIM microscopy, in combination with an Ir(III)-based probe, enables visualization of cellular-level heterogeneity of tumor oxygenation.

摘要

肿瘤中的氧气水平是其发展和对治疗反应的关键因素。利用磷光寿命成像（PLIM）和磷光氧探针是一种高度敏感、非侵入性的光学技术，可用于评估活细胞和组织中的分子氧。在这里，我们提出了一种在体小鼠肿瘤模型中氧分布微观图谱的方案。我们证明，PLIM 显微镜结合基于 Ir(III)的探针，可以实现肿瘤氧合细胞水平异质性的可视化。