Razuvaev Institute of Organometallic Chemistry, Russian Academy of Sciences, Tropinina, 49, 603950 Nizhny Novgorod, Russia.
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Minin and Pozharsky Sq. 10/1, 603005 Nizhny Novgorod, Russia.
Molecules. 2021 Oct 20;26(21):6349. doi: 10.3390/molecules26216349.
New water-soluble polynorbornenes - containing oligoether, amino acid groups and luminophoric complexes of iridium(III) were synthesized by ring-opening metathesis polymerization. The polymeric products in organic solvents and in water demonstrate intense photoluminescence in the red spectral region. The polymers and with 1-phenylisoquinoline cyclometalating ligands in iridium fragments reveal 4-6 fold higher emission quantum yields in solutions than those of and that contain iridium complexes with 1-(thien-2-yl)isoquinoline cyclometalating ligands. The emission parameters of - in degassed solutions essentially differ from those in the aerated solutions showing oxygen-dependent quenching of phosphorescence. Biological testing of and demonstrates that the polymers do not penetrate into live cultured cancer cells and normal skin fibroblasts and do not possess cytotoxicity within the concentrations and time ranges reasonable for biological studies. In vivo, the polymers display longer phosphorescence lifetimes in mouse tumors than in muscle, as measured using phosphorescence lifetime imaging (PLIM), which correlates with tumor hypoxia. Therefore, preliminary evaluation of the synthesized polymers shows their suitability for noninvasive in vivo assessments of oxygen levels in biological tissues.
新型水溶性聚降冰片烯 - 含有聚醚、氨基酸基团和铱（III）发光配合物，通过开环复分解聚合合成。在有机溶剂和水中的聚合物产物在红色光谱区域表现出强烈的光致发光。聚合物 和 具有铱片段中的 1-苯基异喹啉环金属化配体，在溶液中的发射量子产率比那些含有 1-（噻吩-2-基）异喹啉环金属化配体的铱配合物的 高出 4-6 倍。 在真空溶液中的发射参数与充气溶液中的发射参数有很大的不同，表明磷光的氧依赖性猝灭。 和 的生物学测试表明，聚合物不会穿透活培养的癌细胞和正常皮肤成纤维细胞，并且在合理的生物研究浓度和时间范围内不具有细胞毒性。在体内，使用磷光寿命成像（PLIM）测量时，聚合物在小鼠肿瘤中的磷光寿命比肌肉中的磷光寿命长，这与肿瘤缺氧有关。因此，对合成聚合物的初步评估表明它们适合用于生物组织中氧水平的非侵入性体内评估。
