Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
Department of Cell and Tissues, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
Cells Tissues Organs. 2024;213(5):368-381. doi: 10.1159/000537688. Epub 2024 Feb 6.
Ocular diseases pose a significant health concern for donkeys. However, studies examining the microanatomy and cell populations of the donkey retina are scarce. The current study aimed to describe the vascular pattern of the donkey retina and document its cellular components.
The donkey retina specimens were obtained from different retinal regions and prepared for semithin sectioning and immunohistochemistry.
The donkey has a paurangiotic retina in which retinal vessels are confined to a narrow area around the optic disc. Glial cells coexist with the blood vessels being very numerous in the vascular region and become scanty in the avascular ones. S-100-positive astrocytes could be observed in these avascular areas. Ganglion cells are organized in a single layer with the least population existing in the peripheral retina. Acidic fibroblast growth factor (AFGF) is immunoreactive in amacrine and ganglion cells. A subpopulation of amacrine cells reacted strongly to tyrosine hydroxylase (TH), and others reacted positively to S-100 protein. Ganglion cell nuclei exhibited a strong immunoreactivity to S-100 protein as well. Furthermore, glial fibrillary acidic protein (GFAP) is used to identify Müller cells that extend their processes across the retina from the inner to the outer limiting membrane.
In conclusion, our findings provide novel insights into the normal retinal organization. The donkey retina shows the characteristic expression of immunohistochemical markers for the major cell types. In addition, the distribution of glial cells is comparable between the vascular and avascular regions.
眼部疾病是驴的重大健康问题。然而，研究驴视网膜的微观解剖结构和细胞群的研究很少。本研究旨在描述驴视网膜的血管模式，并记录其细胞成分。
从不同的视网膜区域获得驴视网膜标本，并准备进行半薄切片和免疫组织化学。
驴具有假性双血管视网膜，视网膜血管仅限于视神经盘周围的狭窄区域。神经胶质细胞与血管共存，在血管区域非常多，在无血管区域则稀少。在这些无血管区域可以观察到 S-100 阳性的星形胶质细胞。神经节细胞组织成单层，最外层的神经节细胞数量最少。酸性成纤维细胞生长因子（AFGF）在无长突细胞和神经节细胞中具有免疫反应性。一部分无长突细胞对酪氨酸羟化酶（TH）强烈反应，而其他细胞对 S-100 蛋白呈阳性反应。神经节细胞核对 S-100 蛋白也表现出强烈的免疫反应性。此外，胶质纤维酸性蛋白（GFAP）用于鉴定从内界膜到外界膜延伸其过程的 Müller 细胞。
总之，我们的发现为正常视网膜组织提供了新的见解。驴视网膜显示出主要细胞类型的免疫组织化学标志物的特征表达。此外，血管区和无血管区的神经胶质细胞分布相似。
