Clinical Mass Spectrometry Laboratory of Clinical Research Institute and Department of Basic Medicine of Nuclear Industrial Hygiene School, Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, China.
School of Public Health, University of South China, Hengyang, China.
Hum Exp Toxicol. 2024 Jan-Dec;43:9603271241232609. doi: 10.1177/09603271241232609.
Adipose tissue is the main organ that stores lipids and it plays important roles in metabolic balance in the body. We recently reported in Human and Experimental Toxicology that the combined exposure to BPA and fructose may interfere with energy metabolism of adipose tissue. However, it is still unclear whether the combined exposure to BPA and fructose has the possibility to induce lipid remodeling in adipose tissue. In the present study, we performed a widely targeted quantitative lipidomic analysis of the adipose tissue of rats after 6 months of BPA and fructose combined exposure. We totally determined 734 lipid molecules in the adipose tissue of rats. Principal component analysis (PCA) showed the group of the combined exposure to higher-dose (25 μg/kg every other day) BPA and fructose can be distinguished from the groups of control, higher-dose BPA exposure and fructose exposure clearly. Partial least squares-discriminant analysis (PLS-DA) and univariate statistical analysis displayed lipids of PC(18:0_ 20:3), TG(8:0_14:0_16:0), TG(12:0_14:0_16:1), TG(10:0_16:0_16:1), TG(12:0_ 14:0_18:1), TG(14:0_ 16:0_16:1), TG(14:0_14:1_16:1), TG(8:0_ 16:1_16:2), TG(14:1_16:1_ 16:1), TG(16:1_18:1_18:1), TG(16:0_16:1_20:4) and TG(15:0_18:1_ 24:1) may contributed the most to the discrimination. These findings indicated that combined exposure to BPA and fructose has the potential to cause lipid remodeling in adipose tissue.
脂肪组织是储存脂质的主要器官，在体内代谢平衡中发挥着重要作用。我们最近在《人类与实验毒理学》杂志上报道，双酚 A（BPA）和果糖的联合暴露可能会干扰脂肪组织的能量代谢。然而，BPA 和果糖的联合暴露是否有可能诱导脂肪组织中的脂质重塑仍不清楚。在本研究中，我们对大鼠脂肪组织进行了广泛靶向的定量脂质组学分析，以评估 BPA 和果糖联合暴露 6 个月后的情况。我们总共确定了大鼠脂肪组织中的 734 种脂质分子。主成分分析（PCA）表明，高剂量（25μg/kg，每隔一天）BPA 和果糖联合暴露组与对照组、高剂量 BPA 暴露组和果糖暴露组明显区分开来。偏最小二乘判别分析（PLS-DA）和单变量统计分析显示，PC（18:0_20:3）、TG（8:0_14:0_16:0）、TG（12:0_14:0_16:1）、TG（10:0_16:0_16:1）、TG（12:0_14:0_18:1）、TG（14:0_16:0_16:1）、TG（14:0_14:1_16:1）、TG（8:0_16:1_16:2）、TG（14:1_16:1_16:1）、TG（16:1_18:1_18:1）、TG（16:0_16:1_20:4）和 TG（15:0_18:1_24:1）可能是导致这种区分的主要脂质。这些发现表明，BPA 和果糖的联合暴露有可能导致脂肪组织中的脂质重塑。
