一种用于免疫荧光显微镜检查的圣路易斯脑炎病毒制剂灭活的简单方法。
A simple method for the inactivation of St. Louis encephalitis virus preparations for immunofluorescent microscopy.
作者信息
Yabrov A, Artsob H, Spence L
出版信息
Can J Microbiol. 1978 Jan;24(1):72-4. doi: 10.1139/m78-014.
DOI:10.1139/m78-014
PMID:383247
Abstract
A study was made of different treatments for the inactivation of St. Louis encephalitis virus in smears prepared for immunofluorescence microscopy. Treatment of infected cells with 0.3% betapropiolactone at 56 degrees C for 40 min resulted in an inactivated virus suitable for immunofluorescence studies.
摘要
对用于免疫荧光显微镜检查的涂片进行了关于圣路易斯脑炎病毒灭活的不同处理研究。在56摄氏度下用0.3%的β-丙内酯处理感染细胞40分钟，可得到适合免疫荧光研究的灭活病毒。
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