Pallangyo K J, Suzuki H, Fukumoto Y, Matsumoto K
Tohoku J Exp Med. 1985 Dec;147(4):349-56. doi: 10.1620/tjem.147.349.
A one point dilution enzyme-linked immunosorbent assay (ELISA) procedure suitable for determining immunoglobulin G (IgG) antibody levels to Toxoplasma gondii (T. gondii) in community seroepidemiological surveys is described. A two-fold serial dilution ELISA procedure was first used to determine the IgG titers in 56 and 83 sera earlier screened by the Sabin-Feldman dye test (DT) and the indirect hemagglutination test (IHA), respectively. The regression rate of the results by the DT and ELISA was 0.92. Comparison of the results by the IHA and the two-fold serial dilution ELISA gave regression coefficient of 0.92. Using the absorbance values for the test sera at dilutions of 1:20, standard curves made by plotting the optical density versus the corresponding dilution factor of a control sera were used to estimate the antibody levels. The regression coefficient of the results by the two-fold serial dilution method and those by the curves for sera with titers of up to 1:320 was 0.97. The curves could not, however, estimate accurately the antibody level in sera with titers above 1:320. The one point dilution ELISA described is a useful epidemiological tool for the screening of IgG antibody to Toxoplasma gondii in the community. However, larger series are required to confirm our observations.
描述了一种适用于社区血清流行病学调查中测定弓形虫免疫球蛋白G（IgG）抗体水平的单点稀释酶联免疫吸附测定（ELISA）方法。首先采用两倍连续稀释ELISA方法分别测定了56份和83份先前经Sabin-Feldman染色试验（DT）和间接血凝试验（IHA）筛选的血清中的IgG滴度。DT和ELISA结果的回归率为0.92。IHA和两倍连续稀释ELISA结果的比较得出回归系数为0.92。利用1:20稀释度下检测血清的吸光度值，通过绘制光密度与对照血清相应稀释倍数的标准曲线来估计抗体水平。两倍连续稀释法与滴度高达1:320血清曲线结果的回归系数为0.97。然而，这些曲线无法准确估计滴度高于1:320血清中的抗体水平。所描述的单点稀释ELISA是社区中筛选弓形虫IgG抗体的一种有用的流行病学工具。然而，需要更大规模的系列研究来证实我们的观察结果。
