Key Laboratory of Animal Medicine in Sichuan Province, Southwest Minzu University, Chengdu 610041, China.
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Sci Total Environ. 2024 Apr 1;919:170699. doi: 10.1016/j.scitotenv.2024.170699. Epub 2024 Feb 5.
During feeding process in intensive chicken farms, the prolonged exposure of chickens to elevated level of ammonia leads to substantial economic losses within poultry farming industry. Luteolin (Lut), known as its anti-inflammatory and antioxidant properties, possesses the ability to eliminate free radicals and enhance the activities of antioxidant enzymes, thus rendering it highly esteemed in production. The objective of this study was to examine the effects of Lut on antioxidant and anti-inflammatory responses of chicken splenic lymphocytes exposed to ammonia. In order to achieve this, we have replicated a protective model involving Lut against ammonia exposure in chicken splenic lymphocytes. The findings of the study indicated that Lut mitigated the elevation of lactate dehydrogenase (LDH), malondialdehyde (MDA), and reactive oxygen species (ROS) induced by ammonia poisoning. Additionally, Lut demonstrated an increase in the expression of antioxidant enzymes, namely superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Furthermore, Lut exhibited a protective effect on cell morphology and ultrastructure following exposure to ammonia. Moreover, Lut exhibited a reduction in the expression of heat shock proteins (HSPs) and inflammatory cytokines, which were found to be highly expressed in splenic lymphocytes after ammonia exposure. Additionally, Lut demonstrated the ability to inhibit the overexpression of pyroptosis-related genes and proteins (NLRP3 and Caspase-1) in splenic lymphocytes following ammonia exposure. Lut exerted an antioxidant effect on lymphocytes, counteracting elevated levels of oxidative stress following exposure to ammonia. Additionally, Lut had the potential to modulate the expression of HSPs, suppressed the inflammatory response subsequent to ammonia exposure, and influenced the expression of NLRP3 and Caspase-1, thereby mitigating pyroptosis induced by ammonia exposure. The exploration of this subject matter can elucidate the protective properties of Lut against NHCl-induced damage in chicken splenic lymphocytes, while also offer insights and experimental groundwork for the utilization of natural therapeutics in animal husbandry to prevent and treat ammonia-related conditions.
在集约化养鸡场的饲养过程中，鸡暴露在高水平氨中时间过长会导致家禽养殖业的重大经济损失。木犀草素（Lut）具有抗炎和抗氧化特性，能够消除自由基并增强抗氧化酶的活性，因此在生产中备受推崇。本研究的目的是研究 Lut 对暴露于氨的鸡脾淋巴细胞的抗氧化和抗炎反应的影响。为了实现这一目标，我们复制了一个涉及 Lut 对抗氨暴露的鸡脾淋巴细胞的保护模型。研究结果表明，Lut 减轻了氨中毒引起的乳酸脱氢酶（LDH）、丙二醛（MDA）和活性氧（ROS）的升高。此外，Lut 还增加了抗氧化酶，即超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的表达。此外，Lut 对暴露于氨后的细胞形态和超微结构表现出保护作用。此外，Lut 降低了 HSPs 和炎症细胞因子的表达，这些因子在氨暴露后在脾淋巴细胞中高度表达。此外，Lut 能够抑制暴露于氨后脾淋巴细胞中与细胞焦亡相关的基因和蛋白质（NLRP3 和 Caspase-1）的过度表达。Lut 对淋巴细胞发挥抗氧化作用，对抗暴露于氨后氧化应激的升高。此外，Lut 有可能调节 HSPs 的表达，抑制氨暴露后的炎症反应，并影响 NLRP3 和 Caspase-1 的表达，从而减轻氨暴露引起的细胞焦亡。对这一主题的探讨可以阐明 Lut 对鸡脾淋巴细胞中 NHCl 诱导损伤的保护作用，并为在畜牧业中利用天然疗法预防和治疗与氨有关的疾病提供见解和实验基础。
