Jiangsu Provincial Agricultural Green and Low Carbon Production Technology Engineering Research Center, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, Jiangsu Province, China.
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou City, Jiangsu Province, China.
PeerJ. 2024 Feb 5;12:e16898. doi: 10.7717/peerj.16898. eCollection 2024.
is a soil-borne pathogenic bacterium that causes crown gall disease in many plants. Chemotaxis offers the ability to find its host and establish infection. Being an aerobic bacterium, possesses one chemotaxis system with multiple potential chemoreceptors. Chemoreceptors play an important role in perceiving and responding to environmental signals. However, the studies of chemoreceptors in remain relatively restricted. Here, we characterized a cytoplasmic chemoreceptor of C58 that contains an N-terminal globin domain. The chemoreceptor was designated as Atu1027. The deletion of Atu1027 not only eliminated the aerotactic response of to atmospheric air but also resulted in a weakened chemotactic response to multiple carbon sources. Subsequent site-directed mutagenesis and phenotypic analysis showed that the conserved residue His100 in Atu1027 is essential for the globin domain's function in both chemotaxis and aerotaxis. Furthermore, deleting Atu1027 impaired the biofilm formation and pathogenicity of . Collectively, our findings demonstrated that Atu1027 functions as an aerotaxis receptor that affects agrobacterial chemotaxis and the invasion of into its host.
是一种土壤传播的病原菌，可引起许多植物的冠瘿病。趋化性使它能够找到宿主并建立感染。作为一种需氧细菌，拥有一个具有多个潜在化学感受器的趋化系统。化学感受器在感知和响应环境信号方面起着重要作用。然而，关于 的化学感受器的研究仍然相对有限。在这里，我们对 C58 的一种细胞质趋化受体进行了表征，该受体包含一个 N 端球蛋白结构域。该趋化受体被命名为 Atu1027。Atu1027 的缺失不仅消除了 对大气空气的趋气反应，而且导致其对多种碳源的趋化反应减弱。随后的定点突变和表型分析表明，Atu1027 中的保守残基 His100 对于球蛋白结构域在趋化性和趋气性中的功能都是必需的。此外，删除 Atu1027 会损害 的生物膜形成和致病性。总的来说，我们的研究结果表明，Atu1027 作为一种趋气受体，影响根瘤农杆菌的趋化性和对其宿主的侵袭。
