Li Shouming, Wang Ying, Liu Yuanyuan, Liu Changhao, Xu Wei, Lu Yongen, Ye Zhibiao
Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization (Xinjiang Production and Construction Crops), College of Agriculture, Shihezi University, Shihezi, China.
National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, China.
Front Plant Sci. 2024 Jan 25;14:1324401. doi: 10.3389/fpls.2023.1324401. eCollection 2023.
Tomatoes are susceptible to damage from cold temperatures in all stages of growth. Therefore, it is important to identify genetic resources and genes that can enhance tomato's ability to tolerate cold. In this study, a population of 223 tomato accessions was used to identify the sensitivity or tolerance of plants to cold stress. Transcriptome analysis of these accessions revealed that , a member of the sucrose synthase gene family, was induced by cold stress. We further investigated the role of in cold stress by overexpression (OE) and RNA interference (RNAi). Compared with the wild type, -OE lines accumulated less MDA and electrolyte leakage and more proline and soluble sugar, maintained higher activities of SOD and CAT, reduced superoxide radicals, and suffered less membrane damage under cold. Thus, our findings indicate that plays a crucial role in the response to cold stress. This study indicates that may serve as a direct target for genetic engineering and improvement projects, which aim to augment the cold tolerance of tomato crops.
番茄在生长的各个阶段都易受低温伤害。因此，识别能够提高番茄耐冷能力的遗传资源和基因非常重要。在本研究中，使用了一个由223份番茄种质组成的群体来鉴定植株对冷胁迫的敏感性或耐受性。对这些种质的转录组分析表明，蔗糖合酶基因家族的一个成员受冷胁迫诱导。我们通过过表达（OE）和RNA干扰（RNAi）进一步研究了该基因在冷胁迫中的作用。与野生型相比，该基因过表达株系积累的丙二醛和电解质渗漏较少，脯氨酸和可溶性糖较多，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性较高，超氧自由基减少，在低温下膜损伤较小。因此，我们的研究结果表明该基因在对冷胁迫的响应中起关键作用。本研究表明，该基因可作为旨在增强番茄作物耐冷性的基因工程和改良项目的直接靶点。
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