Gong Chao, Wang Zhenshuo, Li Zhiliang, Sun Baojuan, Luo Wenlong, Luo Shanwei, Chen Shuting, Mai Peiting, Li Zhenxing, Li Ye, Wang Yikui, Li Tao
Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangzhou, 510640, China.
Department of Plant Pathology, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Hortic Res. 2023 Dec 19;11(2):uhad272. doi: 10.1093/hr/uhad272. eCollection 2024 Feb.
Resistant crop cultivars can recruit beneficial rhizobacteria to resist disease. However, whether this recruitment is regulated by quantitative trait loci (QTL) is unclear. The role of QTL in recruiting specific bacteria against bacterial wilt (BW) is an important question of practical significance to disease management. Here, to identify QTL controlling BW resistance, Super-BSA was performed in F plants derived from resistant eggplant cultivar R06112 × susceptible cultivar S55193. The QTL was narrowed down through BCF-BCF individuals by wilting symptoms and KASP markers. Rhizosphere bacterial composition of R06112, S55193, and resistant individuals EB158 (with the QTL) and susceptible individuals EB327 (without QTL) from BCF generation were assessed by Illumina sequencing-based analysis, and the activation of plant immunity by the bacterial isolates was analyzed. Evidence showed that BW-resistant is controlled by one QTL located at the 270 kb region on chromosome 10, namely , and as candidate genes confirmed by RNA-Seq. has a significant effect on rhizobacteria composition and significantly recruits . pp. A SynCom of three isolated . pp trains significantly reduced the disease incidence, changed activities of CAT, PPO, and PAL and concentration of NO, HO, and O, activated SA and JA signaling-dependent ISR, and displayed immune activation against in eggplant. Our findings demonstrate for the first time that the QTL can recruit beneficial rhizobacteria, which jointly promote the suppression of BW. This method charts a path to develop the QTL in resistant cultivar-driven probiotics to ameliorate plant diseases.
抗性作物品种可以招募有益根际细菌来抵抗疾病。然而，这种招募是否受数量性状基因座（QTL）调控尚不清楚。QTL在招募特定细菌对抗青枯病（BW）方面的作用是疾病管理中一个具有重要实际意义的问题。在此，为了鉴定控制BW抗性的QTL，在由抗性茄子品种R06112与感病品种S55193杂交得到的F植株中进行了超级分离群体分析法（Super-BSA）。通过萎蔫症状和竞争性等位基因特异性PCR（KASP）标记，利用回交一代-回交一代（BCF-BCF）个体将该QTL定位区间缩小。通过基于Illumina测序的分析评估了R06112、S55193以及来自BCF世代的抗性个体EB158（具有该QTL）和感病个体EB327（不具有QTL）的根际细菌组成，并分析了细菌分离物对植物免疫的激活作用。证据表明，BW抗性由位于10号染色体上270 kb区域的一个QTL控制，即，以及通过RNA测序确定的候选基因。对根际细菌组成有显著影响，并显著招募。三个分离的。的合成群落（SynCom）显著降低了发病率，改变了过氧化氢酶（CAT）、多酚氧化酶（PPO）和苯丙氨酸解氨酶（PAL）的活性以及一氧化氮（NO）、过氧化氢（HO）和超氧阴离子（O）的浓度，激活了水杨酸（SA）和茉莉酸（JA）信号依赖的诱导系统抗性（ISR），并在茄子中表现出对的免疫激活作用。我们的研究结果首次证明，该QTL可以招募有益根际细菌，共同促进对BW的抑制。该方法为在抗性品种驱动的益生菌中开发QTL以改善植物病害开辟了一条道路。
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