State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, P. R. China.
Adv Healthc Mater. 2024 Jul;13(18):e2303549. doi: 10.1002/adhm.202303549. Epub 2024 May 17.
Periodontitis is a common oral disease accompanied by inflammatory bone loss. The pathological characteristics of periodontitis usually accompany an imbalance in the periodontal immune microenvironment, leading to difficulty in bone regeneration. Therefore, effective treatment strategies are needed to modulate the immune environment in order to treat periodontitis. Here, highly-oriented periodic lamellae poly(ε-caprolactone) electrospun nanofibers (PLN) are developed by surface-directed epitaxial crystallization. The in vitro result shows that the PLN can precisely modulate macrophage polarization toward the M2 phenotype. Macrophages polarized by PLN significantly enhance the migration and osteogenic differentiation of Bone marrow stromal cells. Notably, results suggest that the topographical cues presented by PLN can modulate macrophage polarization by activating YAP, which reciprocally inhibits the NF-κB signaling pathway. The in vivo results indicate that PLN can inhibit inflammatory bone loss and facilitate bone regeneration in periodontitis. The authors' findings suggest that topographical nanofibers with periodic lamellae is a promising strategy for modulating immune environment to treat inflammatory bone loss in periodontitis.
牙周炎是一种常见的口腔疾病，伴有炎症性骨丧失。牙周炎的病理特征通常伴随着牙周免疫微环境的失衡，导致骨再生困难。因此，需要有效的治疗策略来调节免疫环境，以治疗牙周炎。在这里，通过表面导向外延结晶开发了高度取向的周期性层状聚（ε-己内酯）电纺纳米纤维（PLN）。体外结果表明，PLN 可以精确调节巨噬细胞向 M2 表型极化。由 PLN 极化的巨噬细胞显著增强了骨髓基质细胞的迁移和成骨分化。值得注意的是，结果表明，PLN 呈现的形貌线索可以通过激活 YAP 来调节巨噬细胞极化，YAP 反过来抑制 NF-κB 信号通路。体内结果表明，PLN 可以抑制牙周炎中的炎症性骨丢失并促进骨再生。作者的研究结果表明，具有周期性层状形貌的纳米纤维是一种有前途的调节免疫环境以治疗牙周炎中炎症性骨丢失的策略。
