College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountain Region, College of Animal Science, Guizhou University, Guiyang 550025, China.
Cells. 2024 Jan 23;13(3):206. doi: 10.3390/cells13030206.
(1) Background: Inflammatory responses are implicated in embryo implantation, decidualization, pregnancy maintenance and labor. Both embryo implantation and decidualization are essential to successful pregnancy in rodents and primates. S100A6 is involved in inflammation, tumor development, apoptosis and calcium homeostasis. S100A6 is strongly expressed in mouse decidua, but the underlying mechanisms of how S100A6 regulates implantation and decidualization are poorly defined. (2) Methods: Mouse endometrial stromal and epithelial cells are isolated from day 4 pseudopregnant mouse uteri. Both immunofluorescence and Western blotting are used to analyze the expression and localization of proteins. The molecular mechanism is verified in vitro by Western blotting and the quantitative polymerase chain reaction. (3) Results: From days 4 to 8 of pregnancy, S100A6 is specifically expressed in mouse subluminal stromal cells. Blastocyst-derived lactic acid induces AA secretion by activating the luminal epithelial p-cPLA2. The epithelial AA induces stromal S100A6 expression through the COX2/PGI2/PPAR δ pathway. Progesterone regulates S100A6 expression through the progesterone receptor (PR). S100A6/RAGE signaling can regulate decidualization via EGFR/ERK1/2 in vitro. (4) Conclusions: S100A6, as an inflammatory mediator, is important for mouse implantation and decidualization.
（1）背景：炎症反应与胚胎着床、蜕膜化、妊娠维持和分娩有关。胚胎着床和蜕膜化对于啮齿动物和灵长类动物的成功妊娠都是必不可少的。S100A6 参与炎症、肿瘤发生、细胞凋亡和钙稳态。S100A6 在小鼠蜕膜中强烈表达，但 S100A6 如何调节着床和蜕膜化的潜在机制尚不清楚。（2）方法：从小鼠假孕第 4 天的子宫中分离子宫内膜基质和上皮细胞。采用免疫荧光和 Western blot 分析蛋白的表达和定位。通过 Western blot 和定量聚合酶链反应在体外验证分子机制。（3）结果：从妊娠第 4 天到第 8 天，S100A6 特异性表达于小鼠 subluminal 基质细胞。胚泡衍生的乳酸通过激活腔上皮 p-cPLA2 诱导 AA 分泌。上皮 AA 通过 COX2/PGI2/PPAR δ 途径诱导基质 S100A6 表达。孕酮通过孕激素受体（PR）调节 S100A6 的表达。S100A6/RAGE 信号可以通过 EGFR/ERK1/2 体外调节蜕膜化。（4）结论：作为炎症介质的 S100A6 对小鼠着床和蜕膜化很重要。
Zotero 插件
