State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China.
Yunnan Key Laboratory of Primate Biomedical Research, Kunming 650500, China.
Int J Mol Sci. 2024 Jan 29;25(3):1642. doi: 10.3390/ijms25031642.
Cortical traumatic brain injury (TBI) is a major cause of cognitive impairment accompanied by motor and behavioral deficits, and there is no effective treatment strategy in the clinic. Cell transplantation is a promising therapeutic strategy, and it is necessary to verify the survival and differentiation of cells after transplantation in large animal models like rhesus monkeys. In this study, we transplanted neural stem cells (NSCs) and simultaneously injected basic fibroblast growth factor/epidermal growth factor (bFGF/EGF) into the cortex (visual and sensory cortices) of rhesus monkeys with superficial TBI. The results showed that the transplanted NSCs did not enter the cerebrospinal fluid (CSF) and were confined to the transplantation site for at least one year. The transplanted NSCs differentiated into mature neurons that formed synaptic connections with host neurons, but glial scar formation between the graft and the host tissue did not occur. This study is the first to explore the repairing effect of transplanting NSCs into the superficial cerebral cortex of rhesus monkeys after TBI, and the results show the ability of NSCs to survive long-term and differentiate into neurons, demonstrating the potential of NSC transplantation for cortical TBI.
皮质创伤性脑损伤（TBI）是认知障碍的主要原因，伴有运动和行为缺陷，临床上尚无有效的治疗策略。细胞移植是一种很有前途的治疗策略，有必要在恒河猴等大动物模型中验证移植后细胞的存活和分化。在这项研究中，我们将神经干细胞（NSCs）移植到 TBI 恒河猴的皮质（视觉和感觉皮质）中，并同时注入碱性成纤维细胞生长因子/表皮生长因子（bFGF/EGF）。结果表明，移植的 NSCs 不会进入脑脊液（CSF），并至少在移植部位保留一年。移植的 NSCs 分化为成熟的神经元，与宿主神经元形成突触连接，但移植物和宿主组织之间没有胶质瘢痕形成。这项研究首次探讨了 TBI 后将 NSCs 移植到恒河猴大脑皮质表面的修复效果，结果表明 NSCs 具有长期存活和分化为神经元的能力，证明了 NSC 移植治疗皮质 TBI 的潜力。
