Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs, H-7624 Pécs, Hungary.
Department of Anatomy, Medical School and Research Group for Mood Disorders, University of Pécs, H-7624 Pécs, Hungary.
Int J Mol Sci. 2024 Feb 1;25(3):1765. doi: 10.3390/ijms25031765.
We have previously proven the involvement of transient receptor potential ankyrin 1 (TRPA1) in stress adaptation. A lack of TRPA1 affects both urocortin 1 (member of the corticotropin-releasing hormone (CRH) family) content of the Edinger-Westphal nucleus. The noradrenergic locus ceruleus (LC) is also an important player in mood control. We aimed at investigating whether the TRPA1 is expressed in the LC, and to test if the response to chronic variable mild stress (CVMS) is affected by a lack of TRPA1. The TRPA1 expression was examined via RNAscope in situ hybridization. We investigated TRPA1 knockout and wildtype mice using the CVMS model of depression. Tyrosine hydroxylase (TH) and FOSB double immunofluorescence were used to test the functional neuromorphological changes in the LC. No TRPA1 expression was detected in the LC. The TH content was not affected by CVMS exposure. The CVMS-induced FOSB immunosignal did not co-localize with the TH neurons. TRPA1 is not expressed in the LC. A lack of functional TRPA1 receptor neither directly nor indirectly affects the TH content of LC neurons under CVMS.
我们之前已经证明了瞬时受体电位锚蛋白 1（TRPA1）在应激适应中的作用。TRPA1 的缺乏会影响脑岛室旁核（Edinger-Westphal nucleus）中的促肾上腺皮质激素释放激素（CRH）家族成员之一的脑啡肽 1（urocortin 1）的含量。蓝斑核（locus ceruleus，LC）中的去甲肾上腺素能神经元也是情绪控制的重要参与者。我们旨在研究 TRPA1 是否在 LC 中表达，并测试缺乏 TRPA1 是否会影响慢性可变轻度应激（chronic variable mild stress，CVMS）的反应。通过 RNAscope 原位杂交检测 TRPA1 的表达。我们使用抑郁症的 CVMS 模型研究了 TRPA1 敲除和野生型小鼠。酪氨酸羟化酶（tyrosine hydroxylase，TH）和 FOSB 双重免疫荧光用于测试 LC 中的功能神经形态变化。LC 中未检测到 TRPA1 的表达。TH 含量不受 CVMS 暴露的影响。CVMS 诱导的 FOSB 免疫信号与 TH 神经元不共定位。TRPA1 不在 LC 中表达。缺乏功能性 TRPA1 受体不会直接或间接影响 CVMS 下 LC 神经元的 TH 含量。
