Department of Biological & Biomedical Sciences, North Carolina Central University, Durham, NC 27707, USA.
Biomanufacturing Research Institute and Technology Enterprise (BRITE), North Carolina Central University, Durham, NC 27707, USA.
Int J Mol Sci. 2024 Feb 1;25(3):1785. doi: 10.3390/ijms25031785.
Here we use the SCIREQ InExpose system to simulate a biologically relevant vaping model in mice to investigate the role of calcium signaling in vape-dependent pulmonary disease as well as to investigate if there is a gender-based difference of disease. Male and female mice were vaped with JUUL Menthol (3% nicotine) using the SCIREQ InExpose system for 2 weeks. Additionally, 2-APB, a known calcium signaling inhibitor, was administered as a prophylactic for lung disease and damage caused by vaping. After 2 weeks, mice were exposed to lipopolysaccharide (LPS) to mimic a bacterial infection. Post-infection (24 h), mice were sacrificed, and bronchoalveolar lavage fluid (BALF) and lungs were taken. Vaping primed the lungs for worsened disease burden after microbial challenge (LPS) for both males and females, though females presented increased neutrophilia and inflammatory cytokines post-vape compared to males, which was assessed by flow cytometry, and cytokine and histopathological analysis. This increased inflammatory burden was controlled by calcium signaling inhibition, suggesting that calcium dysregulation may play a role in lung injury caused by vaping in a gender-dependent manner.
在这里，我们使用 SCIREQ InExpose 系统在小鼠中模拟与生物学相关的蒸气吸烟模型，以研究钙信号在蒸气依赖型肺部疾病中的作用，以及是否存在基于性别的疾病差异。雄性和雌性小鼠使用 SCIREQ InExpose 系统吸食 JUUL Menthol（3%尼古丁）蒸气 2 周。此外，2-APB（一种已知的钙信号抑制剂）作为预防蒸气吸烟引起的肺部疾病和损伤的药物进行给药。2 周后，小鼠暴露于脂多糖（LPS）以模拟细菌感染。感染后（24 小时），处死小鼠，采集支气管肺泡灌洗液（BALF）和肺组织。蒸气吸烟使肺部在微生物挑战（LPS）后更容易发生疾病负担加重，无论是雄性还是雌性小鼠均如此，尽管与雄性相比，雌性小鼠在蒸气吸烟后中性粒细胞和炎症细胞因子增加，通过流式细胞术和细胞因子及组织病理学分析进行评估。钙信号抑制可控制这种炎症负担的增加，这表明钙失调可能以性别依赖的方式在蒸气吸烟引起的肺部损伤中发挥作用。
