Zeeshan Tayyaba, Qamar Zeeshan, Abdul Nishath Sayed, Soman Cristalle, Bamousa Badr, Marrapodi Maria Maddalena, Cicciù Marco, Minervini Giuseppe
Faculty of Dentistry, Department of Oral and Craniofacial Sciences, University Malaya, Kuala Lumpur, Malaysia.
Department O&MFS and Diagnostic Sciences, College of Medicine and Dentistry, Riyadh Elm University, Riyadh, Saudi Arabia.
Technol Health Care. 2024;32(4):2685-2696. doi: 10.3233/THC-231814.
Commercially available oral rinses contain active ingredients with concentration that is claimed by manufacturers to be effective as antiplaque agent. To date there has been no mention of the effect of oral rinse on the adherence of early plaque colonizers in plaque formation and the concentration to be used before/after meals.
The chief aim of the study was to evaluate microbial retention on the salivary pellicle on treatment with oral rinses (CHX & EO)/PS (mimicking after meals use of mouth wash/PS).
Noordini's Artifical Mouth model was used for developing the single species biofilm with early microbial colonizers of oral biofilm (A. viscosus, Strep. mitis and Strep. sanguinis respectively). The microbial retention on use of oral rinses comprising of CHX and EO as an active ingredients respectively was compared with Curcumin PS. For evaluating the microbial retention, the pellicle with microbial inoculation was developed on the glass beads in the mouth model. Subsequently the respective single specie biofilm was exposed to the mouth wash and PS after inoculation. It mimicked as use of mouth wash/PS after meals. The bacterial count in the dental biofilm was evaluated on serial dilution (CFU/ml). Sterile deionized water was used as a negative control. For qualitative analysis, Scanning electron microscope (SEM) was used to evaluate the microbial count.
From the data it was observed that for the treatment of single species experimental biofilm with commercially available mouth rinses (CHX & EO) and PS (curcumin), there was significant retention for S.mitis, S.sanguinis and A.viscosus. There was no significant difference observed between PS and CHX treated single species biofilm. Whereas a significant difference was observed between EO treated biofilms and CHX/PS treated biofilms (p⩽ 0.05).
It can be concluded from the results that curcumin PS and CHX should not be used after meals whereas EO containing mouth rinse can be used to maintain the oral mocroflora.
市售口腔含漱液含有活性成分，制造商宣称其浓度可有效作为抗牙菌斑剂。迄今为止，尚未提及口腔含漱液对早期菌斑定植菌在菌斑形成中的黏附作用以及饭前/饭后使用的浓度。
本研究的主要目的是评估用口腔含漱液（洗必泰&桉叶油）/PS（模拟饭后使用漱口水/PS）处理后唾液薄膜上的微生物留存情况。
使用诺尔迪尼人工口腔模型培养单一菌种生物膜，该生物膜含有口腔生物膜的早期微生物定植菌（分别为黏性放线菌、缓症链球菌和血链球菌）。将分别含有洗必泰和桉叶油作为活性成分的口腔含漱液与姜黄素PS在使用后对微生物留存情况进行比较。为评估微生物留存情况，在口腔模型的玻璃珠上培养接种有微生物的薄膜。随后，在接种后将各自的单一菌种生物膜暴露于漱口水和PS中。这模拟了饭后使用漱口水/PS的情况。通过系列稀释（CFU/ml）评估牙菌斑生物膜中的细菌数量。无菌去离子水用作阴性对照。为进行定性分析，使用扫描电子显微镜（SEM）评估微生物数量。
从数据中观察到，用市售漱口水（洗必泰&桉叶油）和PS（姜黄素）处理单一菌种实验性生物膜时，缓症链球菌、血链球菌和黏性放线菌均有显著留存。在PS和洗必泰处理的单一菌种生物膜之间未观察到显著差异。而在桉叶油处理的生物膜与洗必泰/PS处理的生物膜之间观察到显著差异（p⩽0.05）。
从结果可以得出结论，姜黄素PS和洗必泰不应在饭后使用，而含桉叶油的漱口水可用于维持口腔微生物群落。
