• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

RPA与Rad52相互作用以促进减数分裂交叉和非交叉重组。

RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination.

作者信息

Joo Jeong H, Hong Soogil, Higashide Mika T, Choi Eui-Hwan, Yoon Seobin, Lee Min-Su, Kang Hyun Ah, Shinohara Akira, Kleckner Nancy, Kim Keun P

机构信息

Department of Life Sciences, Chung-Ang University, Seoul 06974, South Korea.

Institute for Protein Research, Graduate School of Science, Osaka University, Osaka 565-0871, Japan.

出版信息

Nucleic Acids Res. 2024 Apr 24;52(7):3794-3809. doi: 10.1093/nar/gkae083.

DOI:10.1093/nar/gkae083
PMID:38340339
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11040155/
Abstract

Meiotic recombination is initiated by programmed double-strand breaks (DSBs). Studies in Saccharomyces cerevisiae have shown that, following rapid resection to generate 3' single-stranded DNA (ssDNA) tails, one DSB end engages a homolog partner chromatid and is extended by DNA synthesis, whereas the other end remains associated with its sister. Then, after regulated differentiation into crossover- and noncrossover-fated types, the second DSB end participates in the reaction by strand annealing with the extended first end, along both pathways. This second-end capture is dependent on Rad52, presumably via its known capacity to anneal two ssDNAs. Here, using physical analysis of DNA recombination, we demonstrate that this process is dependent on direct interaction of Rad52 with the ssDNA binding protein, replication protein A (RPA). Furthermore, the absence of this Rad52-RPA joint activity results in a cytologically-prominent RPA spike, which emerges from the homolog axes at sites of crossovers during the pachytene stage of the meiotic prophase. Our findings suggest that this spike represents the DSB end of a broken chromatid caused by either the displaced leading DSB end or the second DSB end, which has been unable to engage with the partner homolog-associated ssDNA. These and other results imply a close correspondence between Rad52-RPA roles in meiotic recombination and mitotic DSB repair.

摘要

减数分裂重组由程序性双链断裂（DSB）启动。酿酒酵母中的研究表明，在快速切除以产生3'单链DNA（ssDNA）尾巴后，一个DSB末端与同源配对染色单体结合并通过DNA合成进行延伸，而另一端则与其姐妹染色单体保持关联。然后，在调控分化为交叉型和非交叉型命运类型后，第二个DSB末端通过与延伸的第一个末端进行链退火，沿着两条途径参与反应。这种第二个末端的捕获依赖于Rad52，大概是通过其已知的退火两条ssDNA的能力。在这里，我们通过对DNA重组的物理分析表明，这个过程依赖于Rad52与单链DNA结合蛋白复制蛋白A（RPA）的直接相互作用。此外，缺乏这种Rad52-RPA联合活性会导致在减数分裂前期粗线期交叉位点处从同源轴出现细胞学上显著的RPA峰。我们的研究结果表明，这个峰代表了由移位的领先DSB末端或第二个DSB末端导致的断裂染色单体的DSB末端，而第二个DSB末端无法与同源配对相关的ssDNA结合。这些以及其他结果暗示了Rad52-RPA在减数分裂重组和有丝分裂DSB修复中的作用之间存在密切对应关系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/73d4789a2ade/gkae083fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/68b754ac3f13/gkae083figgra1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/b1e12a7a8927/gkae083fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/bd76dfd76b82/gkae083fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/56e89deabc74/gkae083fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/3a7191548013/gkae083fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/01cfb49dfde7/gkae083fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/caba05b85af1/gkae083fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/73d4789a2ade/gkae083fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/68b754ac3f13/gkae083figgra1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/b1e12a7a8927/gkae083fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/bd76dfd76b82/gkae083fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/56e89deabc74/gkae083fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/3a7191548013/gkae083fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/01cfb49dfde7/gkae083fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/caba05b85af1/gkae083fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb3e/11040155/73d4789a2ade/gkae083fig7.jpg

相似文献

1
RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination.RPA与Rad52相互作用以促进减数分裂交叉和非交叉重组。
Nucleic Acids Res. 2024 Apr 24;52(7):3794-3809. doi: 10.1093/nar/gkae083.
2
Mechanism of Rad51 filament formation by Rad52 and Rad55-Rad57 in homologous recombination.Rad52和Rad55-Rad57在同源重组中形成Rad51细丝的机制。
Nat Commun. 2025 Jul 21;16(1):6685. doi: 10.1038/s41467-025-61811-0.
3
Recombination-coupled DNA synthesis facilitates post-invasion steps in meiotic crossover and noncrossover formations.重组偶联DNA合成促进减数分裂交叉和非交叉形成中的入侵后步骤。
Nucleic Acids Res. 2025 Jul 19;53(14). doi: 10.1093/nar/gkaf704.
4
Molecular anatomy of the recombination mediator function of Saccharomyces cerevisiae Rad52.酿酒酵母Rad52重组介导功能的分子剖析
J Biol Chem. 2008 May 2;283(18):12166-74. doi: 10.1074/jbc.M800763200. Epub 2008 Feb 29.
5
Rad52 promotes second-end DNA capture in double-stranded break repair to form complement-stabilized joint molecules.Rad52在双链断裂修复中促进第二末端DNA捕获，以形成互补稳定的接头分子。
Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3077-82. doi: 10.1073/pnas.0813247106. Epub 2009 Feb 9.
6
A large C-terminal Rad52 segment acts as a chaperone to Form and Stabilize Rad51 Filaments.一个大的C端Rad52片段作为伴侣蛋白来形成并稳定Rad51丝。
Nat Commun. 2025 Jul 1;16(1):5589. doi: 10.1038/s41467-025-60664-x.
7
Loss of Meiotic Double Strand Breaks Triggers Recruitment of Recombination-independent Pro-crossover Factors in Spermatogenesis.减数分裂双链断裂的缺失触发精子发生中不依赖重组的前交叉因子的募集。
bioRxiv. 2025 Jun 13:2025.06.10.658785. doi: 10.1101/2025.06.10.658785.
8
Protein dynamics during presynaptic-complex assembly on individual single-stranded DNA molecules.在单个单链 DNA 分子上的突触前复合物组装过程中的蛋白质动力学。
Nat Struct Mol Biol. 2014 Oct;21(10):893-900. doi: 10.1038/nsmb.2886. Epub 2014 Sep 7.
9
Dynamic regulatory interactions of rad51, rad52, and replication protein-a in recombination intermediates.重组中间体中rad51、rad52与复制蛋白A的动态调控相互作用
J Mol Biol. 2009 Jul 3;390(1):45-55. doi: 10.1016/j.jmb.2009.05.009. Epub 2009 May 13.
10
An acidic loop in the forkhead-associated domain of the yeast meiosis-specific kinase Mek1 interacts with a specific motif in a subset of Mek1 substrates.酵母减数分裂特异性激酶 Mek1 的叉头相关结构域中的酸性环与 Mek1 底物的一个亚组中的特定基序相互作用。
Genetics. 2024 Sep 4;228(1). doi: 10.1093/genetics/iyae106.

引用本文的文献

1
Crossover designation recruits condensin to reorganize the meiotic chromosome axis.交叉指定招募凝聚素以重组减数分裂染色体轴。
bioRxiv. 2025 Mar 11:2020.07.16.207068. doi: 10.1101/2020.07.16.207068.
2
Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis.复制蛋白A（RPA）在酵母减数分裂中重组检查点的维持中起关键作用。
Sci Rep. 2024 Apr 25;14(1):9550. doi: 10.1038/s41598-024-60082-x.

本文引用的文献

1
Yeast Rad52 is a homodecamer and possesses BRCA2-like bipartite Rad51 binding modes.酵母 Rad52 是一个同源十四聚体，具有与 BRCA2 类似的二分 Rad51 结合模式。
Nat Commun. 2023 Oct 5;14(1):6215. doi: 10.1038/s41467-023-41993-1.
2
Meiosis: Dances Between Homologs.减数分裂：同源染色体的舞蹈。
Annu Rev Genet. 2023 Nov 27;57:1-63. doi: 10.1146/annurev-genet-061323-044915. Epub 2023 Oct 3.
3
Meiotic prophase roles of Pds5 in recombination and chromosome condensation in budding yeast.减数分裂前期 Pds5 在芽殖酵母重组和染色体浓缩中的作用。
J Microbiol. 2022 Feb;60(2):177-186. doi: 10.1007/s12275-022-1635-9. Epub 2022 Feb 1.
4
Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombination.重复链入侵和广泛的分支迁移是减数分裂重组的标志。
Mol Cell. 2021 Oct 21;81(20):4258-4270.e4. doi: 10.1016/j.molcel.2021.08.003. Epub 2021 Aug 27.
5
The synaptonemal complex central region modulates crossover pathways and feedback control of meiotic double-strand break formation.联会复合体中心区域调节交叉途径和减数分裂双链断裂形成的反馈控制。
Nucleic Acids Res. 2021 Jul 21;49(13):7537-7553. doi: 10.1093/nar/gkab566.
6
The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates.在减数分裂中 RPA、RAD51 和 DMC1 结合的构象揭示了关键重组中间体的本质。
Mol Cell. 2020 Aug 20;79(4):689-701.e10. doi: 10.1016/j.molcel.2020.06.015. Epub 2020 Jun 30.
7
Mechanistic Insight into Crossing over during Mouse Meiosis.鼠减数分裂中交叉现象的机制见解。
Mol Cell. 2020 Jun 18;78(6):1252-1263.e3. doi: 10.1016/j.molcel.2020.04.009. Epub 2020 May 1.
8
Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination.CDK1 通过对 Rad51/Rad52 的磷酸化调控，作为一个分子开关，实现了同源重组在细胞周期中的特异性激活。
Sci Adv. 2020 Feb 7;6(6):eaay2669. doi: 10.1126/sciadv.aay2669. eCollection 2020 Feb.
9
A mutant form of Dmc1 that bypasses the requirement for accessory protein Mei5-Sae3 reveals independent activities of Mei5-Sae3 and Rad51 in Dmc1 filament stability.一种突变形式的 Dmc1 可以绕过辅助蛋白 Mei5-Sae3 的需求，揭示了 Mei5-Sae3 和 Rad51 在 Dmc1 丝稳定性中的独立活性。
PLoS Genet. 2019 Dec 2;15(12):e1008217. doi: 10.1371/journal.pgen.1008217. eCollection 2019 Dec.
10
Crossing and zipping: molecular duties of the ZMM proteins in meiosis.交叉与拉链化：减数分裂中ZMM蛋白的分子功能
Chromosoma. 2019 Sep;128(3):181-198. doi: 10.1007/s00412-019-00714-8. Epub 2019 Jun 25.