Li Shuai, Li Yaling, Zhu Hong, Chen Liyang, Zhang Huiying, Lian Lijie, Xu Miaomiao, Feng Xilong, Hou Rui, Yao Xiaolin, Lin Yifan, Wang Huaying, Wang Xutong
College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Road, Hongshan District, Wuhan, Hubei, 430070, China.
Plant J. 2024 Jun;118(5):1413-1422. doi: 10.1111/tpj.16680. Epub 2024 Feb 11.
Mung bean (Vigna radiata) stands as a crucial legume crop in Asia, contributing to food security. However, our understanding of the underlying genetic foundation governing domesticated agronomic traits, especially those linked to pod architecture, remains largely unexplored. In this study, we delved into the genomic divergence between wild and domesticated mung bean varieties, leveraging germplasm obtained from diverse sources. Our findings unveiled pronounced variation in promoter regions (35%) between the two mung bean subpopulations, suggesting substantial changes in gene expression patterns during domestication. Leveraging transcriptome analysis using distinct reproductive stage pods and subpopulations, we identified candidate genes responsible for pod and seed architecture development, along with Genome-Wide Association Studies (GWAS) and Quantitative Trait Locus (QTL) analysis. Notably, our research conclusively confirmed PDH1 as a parallel domesticated gene governing pod dehiscence in legumes. This study imparts valuable insights into the genetic underpinnings of domesticated agronomic traits in mung bean, and simultaneously highlighting the parallel domestication of pivotal traits within the realm of legume crops.
绿豆（Vigna radiata）是亚洲一种重要的豆类作物，对粮食安全有重要贡献。然而，我们对控制驯化农艺性状，尤其是与豆荚结构相关性状的潜在遗传基础的了解，在很大程度上仍未得到探索。在本研究中，我们利用从不同来源获得的种质，深入研究了野生和驯化绿豆品种之间的基因组差异。我们的研究结果揭示了两个绿豆亚群之间启动子区域存在显著差异（35%），这表明驯化过程中基因表达模式发生了重大变化。利用不同生殖阶段豆荚和亚群的转录组分析，我们确定了负责豆荚和种子结构发育的候选基因，同时进行了全基因组关联研究（GWAS）和数量性状位点（QTL）分析。值得注意的是，我们的研究最终证实PDH1是控制豆类作物豆荚开裂的一个平行驯化基因。本研究为绿豆驯化农艺性状的遗传基础提供了有价值的见解，同时突出了豆类作物领域关键性状的平行驯化。
