Di Ruolin, Zhu Lixiang, Huang Zhen, Lu Minyan, Yin Liuyu, Wang Caixia, Bao Yixue, Duan Zhenzhen, Powell Charles A, Hu Qin, Zhang Jisen, Zhang Muqing, Yao Wei
State Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning 530005, China.
IRREC-IFAS, University of Florida, Fort Pierce, FL 34945, USA.
Gene. 2024 May 20;907:148260. doi: 10.1016/j.gene.2024.148260. Epub 2024 Feb 9.
Pokkah Boeng disease (PBD), caused by Fusarium sacchari, severely affects sugarcane yield and quality. Necrosis-inducing secreted protein 1 (Nis1) is a fungal secreted effector that induces necrotic lesions in plants. It interacts with host receptor-like kinases and inhibits their kinase activity. FsNis1 contains the Nis1 structure and triggered a pathogen-associated molecular pattern-triggered immune response in Nicotiana benthamiana, as reflected by causing reactive oxygen species production, callose accumulation, and the upregulated expression of defense response genes. Knockout of this gene in F. sacchari revealed a significant reduction in its pathogenicity, whereas the pathogenicity of the complementary mutant recovered to the wild-type levels, making this gene an important virulence factor for F. sacchari. In addition, the signal peptide of FsNis1 was required for the induction of cell death and PTI response in N. benthamiana. Thus, FsNis1 may not only be a key virulence factor for F. sacchari but may also induce defense responses in plants. These findings provide new insights into the function of Nis1 in host-pathogen interactions.
赤腐病（PBD）由甘蔗镰刀菌引起，严重影响甘蔗的产量和品质。坏死诱导分泌蛋白1（Nis1）是一种真菌分泌效应蛋白，可在植物中诱导坏死病变。它与宿主类受体激酶相互作用并抑制其激酶活性。FsNis1含有Nis1结构，并在本氏烟草中引发了病原体相关分子模式触发的免疫反应，表现为产生活性氧、胼胝质积累以及防御反应基因的表达上调。在甘蔗镰刀菌中敲除该基因后，其致病性显著降低，而互补突变体的致病性恢复到野生型水平，这使得该基因成为甘蔗镰刀菌的一个重要毒力因子。此外，FsNis1的信号肽是在本氏烟草中诱导细胞死亡和PTI反应所必需的。因此，FsNis1不仅可能是甘蔗镰刀菌的关键毒力因子，还可能诱导植物的防御反应。这些发现为Nis1在宿主-病原体相互作用中的功能提供了新的见解。
