Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Perm, Russia.
Bull Exp Biol Med. 2024 Jan;176(3):354-358. doi: 10.1007/s10517-024-06023-0. Epub 2024 Feb 12.
The influence of SARS-CoV-2 antigen on the cytokine-producing function of immune cells was studied. We observed suppression of the production of proinflammatory cytokines by 11-46% relative to the spontaneous level under the influence of SARS-CoV-2 antigen vaccine simulator, as well as when it was co-administered with cortisol (IL-6 by 1.8 times and IFNγ by 1.57 times) compared with control samples. IL-8 production was reduced by 1.72 times relative to its spontaneous level. IL-8 production was reduced by 1.72 times relative to its spontaneous level. Under conditions of SARS-CoV-2 stimulation with the vaccine antigen in vitro, an increase in the relative scaled expression of the VEGFA gene by 2.16 times relative to the spontaneous level was observed, which can be regarded as a model "cytokine storm" scenario. The obtained experimental data verify the ideas about the pathogenetic mechanisms of the COVID-19 and can contribute to the development of new approaches to the correction of its complications.
研究了 SARS-CoV-2 抗原对免疫细胞产生细胞因子功能的影响。我们观察到，在 SARS-CoV-2 抗原疫苗模拟器的影响下，与对照样本相比，细胞因子的产生被抑制了 11-46%，相对于自发水平；当与皮质醇（IL-6 增加 1.8 倍，IFNγ 增加 1.57 倍）同时给药时也是如此。与自发水平相比，IL-8 的产生减少了 1.72 倍。在体外用疫苗抗原刺激 SARS-CoV-2 的情况下，与自发水平相比，VEGFA 基因的相对标度表达增加了 2.16 倍，可以将其视为一种“细胞因子风暴”的模型场景。所获得的实验数据验证了关于 COVID-19 发病机制的想法，并有助于开发纠正其并发症的新方法。
