Digital PCR-based methylation testing in acute myeloid leukemia: diagnosis, prognosis and monitoring.

Acute myeloid leukemia (AML) is a challenging disease with high rates of recurrence. The role of the cancer-related gene in AML has not been widely studied. Peripheral blood samples were collected from 73 AML patients and 68 healthy controls. Droplet digital PCR was used to detect methylation levels to explore the value of methylation in the diagnosis, treatment response and prognosis of AML. methylation was significantly increased in AML patients (p < 0.01), with high diagnostic accuracy (area under the curve: 0.848; p < 0.001). methylation was correlated with chemotherapy response (p < 0.05) and is an independent prognostic factor for AML (p < 0.05). methylation is expected to serve as a biomarker for diagnosing AML patients and predicting prognosis.