Kretschmer Rafael, Santos de Souza Marcelo, Gunski Ricardo José, Del Valle Garnero Analía, de Freitas Thales Renato Ochotorena, Zefa Edison, Toma Gustavo Akira, Cioffi Marcelo de Bello, Herculano Corrêa de Oliveira Edivaldo, O'Connor Rebecca E, Griffin Darren K
School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK.
Departamento de Ecologia, Zoologia e Genética, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas 96010-900, RS, Brazil.
Genome. 2024 Jun 1;67(6):168-177. doi: 10.1139/gen-2023-0101. Epub 2024 Feb 12.
The Cuculiformes are a family of over 150 species that live in a range of habitats, such as forests, savannas, and deserts. Here, bacterial artificial chromosome (BAC) probes (75 from chicken and 14 from zebra finch macrochromosomes 1-10 +ZW and for microchromosomes 11-28 (except 16)) were used to investigate chromosome homologies between chicken and the squirrel cuckoo (). In addition, repetitive DNA probes were applied to characterize the chromosome organization and to explore the role of these sequences in the karyotype evolution of . . We also applied BAC probes for chicken chromosome 17 and Z to the guira cuckoo () to test whether this species has an unusual Robertsonian translocation between a microchromosome and the Z chromosome, recently described in the smooth-billed ani (). Our results revealed extensive chromosome reorganization with inter- and intrachromosomal rearrangements in . , including a conspicuous chromosome size and heterochromatin polymorphism on chromosome pair 20. Furthermore, we confirmed that the Z-autosome Robertsonian translocation found in . is also found in . , not . . These findings suggest that this translocation occurred prior to the divergence between . and . , but after the divergence with . .
杜鹃目是一个拥有超过150个物种的鸟类家族，它们生活在多种栖息地中，如森林、热带稀树草原和沙漠。在这里，细菌人工染色体（BAC）探针（75个来自鸡，14个来自斑胸草雀的1-10号常染色体+ZW染色体以及11-28号微染色体（16号除外））被用于研究鸡与松鼠杜鹃之间的染色体同源性。此外，重复DNA探针被用于表征染色体组织，并探索这些序列在[物种名称]核型进化中的作用。我们还将鸡17号染色体和Z染色体的BAC探针应用于圭拉杜鹃，以测试该物种是否具有最近在光嘴犀鹃中描述的微染色体与Z染色体之间的异常罗伯逊易位。我们的结果揭示了[物种名称]中广泛的染色体重组，包括染色体间和染色体内的重排，其中20号染色体对存在明显的染色体大小和异染色质多态性。此外，我们证实了在[物种名称]中发现的Z-常染色体罗伯逊易位在[物种名称]中也存在，而在[物种名称]中不存在。这些发现表明，这种易位发生在[物种名称]和[物种名称]分化之前，但在与[物种名称]分化之后。
