Campbell G T, Wagoner J, Gregerson K A, Joyner W L
Endocrinology. 1979 Oct;105(4):905-10. doi: 10.1210/endo-105-4-905.
The effects of transplanting neonatal adenohypophyseal tissue into the hamster cheek pouch on the presence of intracellular materials related to PRL in the allografts were examined using immunohistochemistry. In situ pituitary and placental tissue were used as control. Cells with antirat PRL-reactive sites were scarce in the grafts, and radioimmunoassayable serum PRL was not detectable in hypophysectomized hosts with grafts. Antirat GH- and antihuman placental lactogen (hPL)-reactive sites were visualized in the grafts and in situ pituitary tissue. Intracellular material in hamster placental tissue was visualized with anti-hPL only. Results of various immunohistochemical procedures using in situ pituitary tissue and antirat GH and anti-hPL antisera indicated that three cell types could exist: 1) a cell type visualized with only antirat GH, 2) a cell type visualized with only anti-hPL, and 3) a cell type, the most frequently observed, visualized with both antisera. The physiological significance of this intracellular product, which resembles hPL and perhaps hamster PL immunologically, in hamster adenohypophyseal cells is unknown. Additionally, our data indicate that the ectopic site for pituitary transplantation in the hamster may influence the cell types present in the grafts.
采用免疫组织化学方法，研究了将新生腺垂体组织移植到仓鼠颊囊后，同种异体移植物中与催乳素（PRL）相关的细胞内物质的情况。以原位垂体和胎盘组织作为对照。移植物中具有抗大鼠PRL反应位点的细胞稀少，在接受移植物的垂体切除宿主中未检测到可通过放射免疫测定的血清PRL。在移植物和原位垂体组织中可观察到抗大鼠生长激素（GH）和抗人胎盘催乳素（hPL）反应位点。仅用抗hPL可观察到仓鼠胎盘组织中的细胞内物质。使用原位垂体组织以及抗大鼠GH和抗hPL抗血清进行的各种免疫组织化学实验结果表明，可能存在三种细胞类型：1）仅用抗大鼠GH可观察到的细胞类型；2）仅用抗hPL可观察到的细胞类型；3）最常观察到的、用两种抗血清均可观察到的细胞类型。这种在仓鼠腺垂体细胞内的产物在免疫学上类似于hPL，可能也类似于仓鼠PL，其生理意义尚不清楚。此外，我们的数据表明，仓鼠垂体移植的异位部位可能会影响移植物中存在的细胞类型。
