Department of Pathology, Field of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
Methods Mol Biol. 2024;2763:101-109. doi: 10.1007/978-1-0716-3670-1_8.
Immunohistochemistry (IHC) staining is the most common method to detect the distribution and localization of biomarkers in different parts of a tissue. Antibodies for tandem repeat peptide of mucins are very popular, but antibodies for glycosylation or others are also used. IHC for mucin is the same protocol as IHC for others. This description includes IHC according to ABC method for processed formalin-fixed paraffin-embedded (FFPE) tissues. Protocol of in situ hybridization is also shown.
免疫组织化学（IHC）染色是检测组织不同部位生物标志物分布和定位的最常用方法。用于黏蛋白串联重复肽的抗体非常流行，但也使用用于糖基化或其他的抗体。黏蛋白的 IHC 与其他 IHC 的方案相同。此描述包括根据 ABC 方法对福尔马林固定石蜡包埋（FFPE）组织进行的 IHC。原位杂交的方案也显示出来了。
