Aggio M C, Lozzio B B
Exp Hematol. 1979 Apr;7(4):197-205.
The hematopoiesis of athymic-asplenic (lasat) mice was compared with that of normal, asplenic, and athymic littermates with the same strain background. Erythrocyte blood volume, number and survival time were normal when related to the body weight of the animals. Peripheral blood showed leukopenia with absolute and relative lymphopenia, resembling the athymic rather than the asplenic pattern. The bone marrow was hypocellular as a consequence of a decrease in both lymphocytes and erythroid precursors, while thrombocytopoiesis and granulcytopoiesis-monocytopoiesis were essentially normal. Although the percentile value of femoral stem cells was high, their absolute number was, in fact, reduced by 35% as a result of the bone marrow hypocellularity. When lasat bone marrow cells were injected into normal, lethally irradiated mice, a rapid erythropoietic recovery was observed, whereas the restoration of the granlocytic compartment was impaired. It was concluded that: 1) lasat mice depict a normal hematopoiesis in spite of the congenital absence of the thymus and the spleen; 2) bone marrow stem cells may be defective when administered to lethally irradiated hosts; and 3) the athymic status predominates over the asplenic one.
将无胸腺无脾（lasat）小鼠的造血功能与其具有相同品系背景的正常、无脾和无胸腺同窝小鼠进行了比较。与动物体重相关时，红细胞血容量、数量和存活时间均正常。外周血显示白细胞减少，伴有绝对和相对淋巴细胞减少，类似无胸腺而非无脾模式。由于淋巴细胞和红系前体细胞均减少，骨髓细胞减少，而血小板生成和粒细胞生成-单核细胞生成基本正常。尽管股骨干细胞的百分比值较高，但由于骨髓细胞减少，其绝对数量实际上减少了35%。当将lasat骨髓细胞注射到经致死性照射的正常小鼠体内时，观察到红细胞生成迅速恢复，而粒细胞区室的恢复受损。得出以下结论：1）尽管先天性无胸腺和脾脏，lasat小鼠仍表现出正常的造血功能；2）当给予经致死性照射的宿主时，骨髓干细胞可能存在缺陷；3）无胸腺状态比无脾状态占主导。
