Tenenbaum A, Sernvi C, Forsberg J G
Biol Res Pregnancy Perinatol. 1985;6(3):143-9.
Ovaries from 8-week-old female NMRI mice, treated with 5 micrograms diethylstilbestrol (DES) daily for the first 5 days after birth were studied for progesterone synthesis in vitro, using 3H-pregnenolone as precursor, and assayed for progesterone content using radioimmunoassay (RIA) technique. The plasma levels of progesterone were measured with RIA. These results were compared with those from control ovaries from females in different phases of the estrous cycle. Values for radioactivity representing 3H-progesterone were significantly higher after 1-hour incubation of ovarian homogenate from DES treated females than in homogenates of ovaries from any phase of the normal estrous cycle. The ovarian content of progesterone was similar in DES exposed ovaries and ovaries from females in proestrous or estrous but lower in DES ovaries than in diestrous ovaries. The plasma levels of progesterone were in the same range in DES treated females and diestrous females but higher in DES females than in estrous or proestrous females.
对出生后前5天每天接受5微克己烯雌酚（DES）处理的8周龄雌性NMRI小鼠的卵巢进行体外孕酮合成研究，以3H-孕烯醇酮作为前体，并使用放射免疫测定（RIA）技术测定孕酮含量。用RIA测量孕酮的血浆水平。将这些结果与来自处于发情周期不同阶段的雌性对照卵巢的结果进行比较。在DES处理的雌性小鼠卵巢匀浆孵育1小时后，代表3H-孕酮的放射性值显著高于正常发情周期任何阶段卵巢匀浆中的值。DES暴露卵巢和发情前期或发情期雌性卵巢中的孕酮含量相似，但DES处理卵巢中的孕酮含量低于动情间期卵巢。DES处理雌性小鼠和动情间期雌性小鼠的血浆孕酮水平在相同范围内，但DES处理雌性小鼠的血浆孕酮水平高于发情期或发情前期雌性小鼠。
