Laboratório de Ultraestrutura Celular Hertha Meyer, Centro de Pesquisa em Medicina de Precisão, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, Cidade Universitária, Rio de Janeiro, RJ, 21941-904, Brazil.
Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Int Ophthalmol. 2024 Feb 13;44(1):73. doi: 10.1007/s10792-024-02985-2.
To provide a simple alternative acute ocular toxoplasmosis model with great reproducibility for experimental tests that demand monitoring of the ocular lesion.
ME49-wt and ME49-GFP tachyzoites from cell culture were used to infect male C57BL6 mice by intraperitoneal injection. B1 expression by real-time polymerase chain reaction (qPCR) assay was used to detect the presence of T. gondii in ocular tissue at the beginning of the infection. Fluorescence microscopy and histopathology analysis were carried out to assess the evolution of the acute infection up to 20 days in both eyes of infected mice.
All mice infected with the 10 tachyzoites showed B1 expression in the retina of both eyes, in the RPE (retinal pigment epithelium), and choroid structures, after 5 days of infection. Tachyzoites of the ME49-GFP strain were easily detected by fluorescence microscopy in the retina tissue of mice after 5 days post-infection. After 20 days, mice inflammatory cell infiltrates and a disorganized morphology of the retinal laminar architecture were observed.
Infection of C57BL6 mice via intraperitoneal with 10 tachyzoites of the ME49-GFP strain from cell culture is a suitable model for acute ocular toxoplasmosis. This model has great reproducibility in establishing the ocular lesion since day 5 post-infection. This model can be suitable for experimental tests of chemotherapy and the investigation of the role of the immune response on the development of uveitis.
提供一种简单的急性眼弓形虫病替代模型，具有很好的重现性，适用于需要监测眼部病变的实验测试。
使用来自细胞培养的 ME49-wt 和 ME49-GFP 速殖子，通过腹腔注射感染雄性 C57BL6 小鼠。通过实时聚合酶链反应 (qPCR) 检测 B1 表达，以检测感染开始时眼组织中弓形虫的存在。荧光显微镜和组织病理学分析用于评估感染后 20 天内感染小鼠双眼的急性感染演变。
所有感染 10 个速殖子的小鼠在感染后 5 天，均在双眼视网膜、RPE（视网膜色素上皮）和脉络膜结构中显示 B1 表达。感染后 5 天，用荧光显微镜很容易在感染小鼠的视网膜组织中检测到 ME49-GFP 株的速殖子。20 天后，观察到小鼠炎症细胞浸润和视网膜层状结构紊乱。
通过腹腔内感染来自细胞培养的 10 个 ME49-GFP 株速殖子的 C57BL6 小鼠是急性眼弓形虫病的合适模型。自感染后第 5 天开始，该模型在建立眼部病变方面具有很好的重现性。该模型可适用于化疗的实验测试以及对免疫反应在葡萄膜炎发展中的作用的研究。
