Merck & Co., Inc., West Point, Pennsylvania 19486, United States.
Anal Chem. 2024 Feb 27;96(8):3489-3497. doi: 10.1021/acs.analchem.3c05278. Epub 2024 Feb 13.
Selection and characterization of antibodies are critically important in establishing robust immunoassays to support the development efforts of vaccines. Plate-based ELISA can be time- and resource-intensive to select initial antibody clones or characterize downstream resupply lots while providing limited information regarding the binding characteristics of the antibodies beyond concentration-response curves. This work applied the microfluidic Gyrolab to holistically evaluate immunoassay reagents through analyses of concentration-response curves as well as antibody-antigen interactions visualized in column images and affinity estimates. We exploited the automation capability of the Gyrolab to reduce the resources (time, reagents, and scientists) required for screening and evaluating antibody reagents. Using a flexible semi-universal assay format, we compared antibody clones for selection and resupply lots of sera and monoclonal antibodies in a simple "plug-and-play" manner without antibody modifications. We found that the performance of antibodies in the Gyrolab correlated well with the trends observed in traditional ELISAs, while the Gyrolab provided additional advantages over plate-based assays such as column images of antibody binding and affinity measurements.
在建立支持疫苗开发工作的强大免疫分析方法时，抗体的选择和鉴定至关重要。基于平板的 ELISA 可能需要花费大量的时间和资源来选择初始抗体克隆或对后续供应批次进行特征分析，同时提供的有关抗体结合特性的信息有限，仅限于浓度反应曲线。这项工作应用微流控 Gyrolab 通过分析浓度反应曲线以及柱图像中可视化的抗体-抗原相互作用和亲和力估计值，全面评估免疫分析试剂。我们利用 Gyrolab 的自动化功能，减少了筛选和评估抗体试剂所需的资源（时间、试剂和科学家）。使用灵活的半通用检测格式，我们以简单的“即插即用”方式比较了用于选择和供应批次血清和单克隆抗体的抗体克隆，而无需对抗体进行修饰。我们发现，Gyrolab 中抗体的性能与传统 ELISA 观察到的趋势密切相关，而 Gyrolab 相对于平板检测具有额外的优势，例如抗体结合的柱图像和亲和力测量。
