Wageningen Bioveterinary Research, Wageningen University and Research, Lelystad, the Netherlands.
Agence nationale de sécurité sanitaire de l'alimentation de l'environnement et du travail (Anses), Laboratoire de Lyon, Unité Maladies Neurodégénératives, Lyon, France.
Neuropathol Appl Neurobiol. 2024 Feb;50(1):e12963. doi: 10.1111/nan.12963.
CH1641 was discovered in 1970 as a scrapie isolate that was unlike all other classical strains of scrapie isolated so far. We performed bio-assays of CH1641 in mice in order to further characterise this specific isolate.
We inoculated the original CH1641 isolate into ovine and bovine prion protein (PrP) transgenic mice as well as wild-type mice. In addition, we performed cross- and back passages between the various mouse lines to examine if one identical prion strain was isolated in all mouse lines or whether multiple prion strains exist in CH1641.
We report the first successful transmission of CH1641 to wild-type RIII mice and via RIII mice to wild-type VM mice. Unexpectedly, analysis of the protease-resistant prion protein (PrP ) in wild-type mice showed a classical scrapie banding pattern differing from the banding pattern of the original CH1641 isolate. Cross- and back passages of CH1641 between the various mouse lines confirmed that the same prion strain had been isolated in all mouse lines.
The CH1641 isolate consists of a single prion strain but its molecular banding pattern of PrP differs between wild-type mice and PrP transgenic mice. Consequently, molecular banding patterns of PrP should be used with caution in strain typing since they do not solely depend on the properties of the prion strain but also on the host prion protein.
CH1641 于 1970 年被发现，是一种不同于迄今为止分离到的所有其他经典朊病毒株的瘙痒病分离株。我们在小鼠中进行了 CH1641 的生物测定，以进一步表征这种特定的分离株。
我们将原始 CH1641 分离株接种到绵羊和牛朊病毒蛋白（PrP）转基因小鼠以及野生型小鼠中。此外，我们在各种小鼠系之间进行了交叉和回传，以检查是否在所有小鼠系中分离到相同的朊病毒株，或者 CH1641 是否存在多种朊病毒株。
我们首次成功地将 CH1641 传播到野生型 RIII 小鼠，并通过 RIII 小鼠传播到野生型 VM 小鼠。出乎意料的是，对野生型小鼠中的蛋白酶抗性朊病毒蛋白（PrP）的分析显示出一种不同于原始 CH1641 分离株的经典瘙痒病带型。在各种小鼠系之间的 CH1641 的交叉和回传证实，相同的朊病毒株已在所有小鼠系中分离到。
CH1641 分离株由单一的朊病毒株组成，但在野生型小鼠和 PrP 转基因小鼠中，其 PrP 的分子带型不同。因此，在进行菌株分型时应谨慎使用 PrP 的分子带型，因为它们不仅取决于朊病毒株的特性，还取决于宿主朊病毒蛋白。
