Feng Jun, Pan Miao, Zhuang Yuan, Luo Jiayuan, Chen Yong, Wu Yitong, Fei Jiayi, Zhu Yanqi, Xu Zhen, Yuan Zhengan, Chen Min
Shanghai Municipal Center for Diseases Control and Prevention, Shanghai, China.
Microbiol Spectr. 2024 Feb 14;12(3):e0370723. doi: 10.1128/spectrum.03707-23.
Long-term care facilities (LTCFs) for older people play an important and unique role in multidrug-resistant organism transmission. Herein, we investigated the genetic characteristics of mobile colistin resistance gene ()-carrying strains isolated from wastewater of LTCFs in Shanghai. Antimicrobial susceptibility test was carried out by agar dilution methods. Whole-genome sequencing and plasmid sequencing were conducted, and resistance genes and sequence types of colistin in isolates were analyzed. Core genome multilocus sequence typing (cgMLST) analysis was performed by the Ridom SeqSphere+ software. Phylogenetic tree through the maximum likelihood method was constructed by MEGA X. Out of 306 isolates, only 1 named ECSJ33 was found, and the plasmid pECSJ33 from ECSJ33 harbored the gene that was located with 59,080 bp belonging to IncI2 type. The plasmid pECSJ33 was capable of conjugation with an efficiency of 2.9 × 10. Bioinformatic analysis indicated pECSJ33 shared backbone with the previously reported -harboring pHNGDF93 isolated from fish source. Moreover, the cgMLST analysis revealed that ECSJ33 belongs to different lineages from those reported from previous strains but shared high similarity to NCTC11129 in cluster 11. The phylogenetic tree revealed MCR-1 of ECSJ33 in this study was mostly of animal food origin and that they were closely related. Our study firstly reports detection of genome sequence of a multidrug-resistant -harboring ST155 from wastewater of LTCF source in China. The data may prove that the plasmid pECSJ33 belongs to food origin and help to understand the antimicrobial resistance mechanisms and genomic features of colistin resistance under One Health approach.IMPORTANCEOne named ECSJ33 was found from wastewater of a long-term care facility (LTCF) and the plasmid pECSJ33 from ECSJ33 harbored the mobile colistin resistance gene () that was located with 59,080 bp belonging to IncI2 type, which was capable of conjugation with an efficiency of 2.9 × 10. This paper firstly reports an -carrying strain ST155 isolated from LTCF in China. Comparative genomics analysis indicated pECSJ33 shared backbone with the previously reported -harboring pHNGDF93 isolated from fish source. The phylogenetic tree revealed MCR-1 protein of ECSJ33 in this study was mostly of animal food origin and that they were closely related. Therefore, the pECSJ33 could be considered as food-origin transmission -harboring plasmid.
老年人长期护理机构（LTCFs）在多重耐药菌传播中发挥着重要且独特的作用。在此，我们调查了从上海长期护理机构废水中分离出的携带可移动黏菌素耐药基因（）的菌株的遗传特征。采用琼脂稀释法进行药敏试验。进行了全基因组测序和质粒测序，并分析了分离株中黏菌素的耐药基因和序列类型。通过Ridom SeqSphere +软件进行核心基因组多位点序列分型（cgMLST）分析。利用MEGA X通过最大似然法构建系统发育树。在306株分离株中，仅发现1株名为ECSJ33的菌株，来自ECSJ33的质粒pECSJ33携带位于59,080 bp处、属于IncI2型的基因。质粒pECSJ33能够进行接合，接合效率为2.9×10。生物信息学分析表明，pECSJ33与先前报道的从鱼类来源分离出的携带的pHNGDF93具有共同的主干结构。此外，cgMLST分析显示，ECSJ33与先前报道的菌株属于不同的谱系，但与第11簇中的NCTC11129具有高度相似性。系统发育树显示，本研究中ECSJ33的MCR - 1主要来源于动物食品，且它们密切相关。我们的研究首次报道了在中国从长期护理机构来源的废水中检测到携带多重耐药的ST155的基因组序列。这些数据可能证明质粒pECSJ33属于食品来源，并有助于在“同一健康”方法下理解黏菌素耐药的抗菌机制和基因组特征。重要性从一家长期护理机构（LTCF）的废水中发现了一株名为ECSJ33的菌株，来自ECSJ33的质粒pECSJ33携带位于59,080 bp处、属于IncI2型的可移动黏菌素耐药基因（），其能够进行接合，接合效率为2.9×10。本文首次报道了在中国从长期护理机构分离出的携带的菌株ST155。比较基因组学分析表明，pECSJ33与先前报道的从鱼类来源分离出的携带的pHNGDF93具有共同的主干结构。系统发育树显示，本研究中ECSJ33的MCR - 1蛋白主要来源于动物食品，且它们密切相关。因此，pECSJ33可被视为携带的食品来源传播质粒。
