State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Department of Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Sci Total Environ. 2023 Oct 20;896:165203. doi: 10.1016/j.scitotenv.2023.165203. Epub 2023 Jul 3.
The rapid global dissemination of Salmonella enterica sequence type 34 (ST34) has sparked significant concern due to its resistance to critical antimicrobials and its ability to spread across various sectors. In order to investigate the evolution and transmission dynamics of this epidemic clonal lineage, as well as the horizontal transfer of mcr-carrying plasmids within the One Health framework, we conducted a comprehensive genomic epidemiological study. This study focused on the 11 mcr-carrying S. enterica isolates obtained from clinical settings in China, while also considering 2337 publicly available genomes of mcr-carrying S. enterica collected from 20 countries and diverse sources spanning over a 22-year period. Among the mcr-positive Salmonella isolates, ST34 was found to be the predominant lineage, comprising 30.12 % (704/2337) of the total collection. These isolates were identified as either serovar Typhimurium or its monophasic variant, which were obtained from both clinical and non-clinical sources. Phylogeographic analyses traced the global spread of the mcr-carrying ST34 lineage, which was divided into three distinct clusters, with 83.10 % of them carrying mcr-1 or/and mcr-9 genes. Notably, the mcr-1 positive ST34 isolates were primarily found in China (190/298, 63.76 %), with only four from the United States. Conversely, mcr-9 positive ST34 isolates were predominantly identified in the United States (261/293, 89.08 %), while none were observed in China. The mcr-1 positive ST34 isolates was predicted to have originated from clinical sources in United Kingdom, whereas mcr-9 positive ST34 isolates was likely derived from environmental sources in Germany. The most recent common ancestor for mcr-1 and mcr-9 carrying ST34 S. enterica was estimated to have emerged around 1983 and 1951. These findings provided thorough and intuitive insights into the intercontinental spread of mcr-carrying S. enterica ST34 lineage in a One Health context. Ongoing surveillance is crucial for effectively monitoring the worldwide dissemination of this multidrug-resistant high-risk clone.
肠沙门氏菌 34 型（ST34）在全球的迅速传播引起了人们的极大关注，因为它对关键抗菌药物具有耐药性，并且能够在多个领域传播。为了研究这一流行克隆谱系的进化和传播动态，以及在同一健康框架内 mcr 携带质粒的水平转移，我们进行了一项全面的基因组流行病学研究。本研究重点关注从中国临床环境中获得的 11 株 mcr 携带肠沙门氏菌分离株，同时还考虑了 2337 株来自 20 个国家和不同来源的 mcr 携带肠沙门氏菌公开基因组，时间跨度为 22 年。在 mcr 阳性沙门氏菌分离株中，ST34 是主要谱系，占总分离株的 30.12%（704/2337）。这些分离株被鉴定为肠炎沙门氏菌或其单相变体，它们分别来自临床和非临床来源。系统发育地理分析追踪了 mcr 携带 ST34 谱系的全球传播，该谱系分为三个不同的簇，其中 83.10%携带 mcr-1 或/和 mcr-9 基因。值得注意的是，mcr-1 阳性 ST34 分离株主要在中国（190/298，63.76%）发现，而来自美国的只有 4 株。相反，mcr-9 阳性 ST34 分离株主要在美国（261/293，89.08%）发现，而在中国没有发现。mcr-1 阳性 ST34 分离株预测起源于英国的临床来源，而 mcr-9 阳性 ST34 分离株可能源自德国的环境来源。携带 mcr-1 和 mcr-9 的 ST34 肠沙门氏菌的最近共同祖先估计于 1983 年和 1951 年出现。这些发现为在同一健康框架内深入直观地了解 mcr 携带 ST34 肠沙门氏菌谱系的洲际传播提供了依据。持续监测对于有效监测这种多药耐药高危克隆在全球的传播至关重要。
