Afraei Fatemeh, Daneshjou Sara, Dabirmanesh Bahareh
Department of Nanobiotechnology, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
AMB Express. 2024 Feb 14;14(1):23. doi: 10.1186/s13568-024-01677-5.
The bacterial enzyme chondroitinase ABCI (chABCI), which has been isolated from Proteus Vulgaris, is crucial in the treatment of spinal cord injuries. However, due to its short lifespan, the maintenance and clinical application of this enzyme are very constrained. In this study, the immobilization of this enzyme on hydroxyapatite has been carried out and assessed with the aim of enhancing the characteristics and efficiency of chABCI. Hydroxyapatite particles (HAPs) are a potential candidate for drug-delivery carriers because of their excellent biocompatibility, shape controllability, and high adsorption. The use of the nanometer scale allows efficient access to the enzyme's substrate. It demonstrates important biological application capabilities in this way. Field emission gun-scanning electron microscopy (FEG-SEM), X-ray diffraction (XRD), infrared spectroscopy (FT-IR), in vitro release study, and cytotoxicity test were used to characterize the drug nanosystem's properties. According to the findings, electrostatic bindings was formed between charged groups of the enzyme and hydroxyapatite nanoparticles. The results also demonstrated that immobilized chABCI on hydroxyapatite has beneficial properties, such as more manageable drug release, minimal toxicity and side effects, and a high potential to enhance the efficacy of drug delivery and decrease the need for repeated injections.
从普通变形杆菌中分离出的细菌酶软骨素酶ABCI（chABCI）在脊髓损伤治疗中至关重要。然而，由于其寿命较短，这种酶的保存和临床应用受到很大限制。在本研究中，已将该酶固定在羟基磷灰石上并进行评估，目的是增强chABCI的特性和效率。羟基磷灰石颗粒（HAPs）因其优异的生物相容性、形状可控性和高吸附性，是药物递送载体的潜在候选者。使用纳米尺度能够有效接触酶的底物。通过这种方式，它展现出重要的生物应用能力。利用场发射枪扫描电子显微镜（FEG-SEM）、X射线衍射（XRD）、红外光谱（FT-IR）、体外释放研究和细胞毒性试验来表征药物纳米系统的性质。根据研究结果，在酶的带电基团与羟基磷灰石纳米颗粒之间形成了静电结合。结果还表明，固定在羟基磷灰石上的chABCI具有有益特性，如更可控的药物释放、最小的毒性和副作用，以及提高药物递送效果和减少重复注射需求的巨大潜力。
