Dipartimento di Ingegneria Enzo Ferrari, Università degli Studi di Modena e Reggio Emilia, Modena, Italy.
Dipartimento di Chimica e Chimica Industriale, Università di Pisa, Pisa, Italy.
J Biomed Mater Res A. 2019 Dec;107(12):2601-2609. doi: 10.1002/jbm.a.36766. Epub 2019 Aug 14.
Different bioactive glasses (BGs), bioceramics, and their composites were extensively analyzed in terms of biological responsiveness and angiogenic potential. In particular several inorganic materials were considered, namely the widely used 45S5 BG, an experimental BG with low tendency to crystallize, other three experimental BGs doped with strontium and/or magnesium, a commercial hydroxyapatite (HA), and two BG-HA composites (with varying percentages of BG and HA). All these materials were ad hoc prepared and in vitro tested by means of an extensive biological analysis, such as MC3T3-E1 cell viability and proliferation by direct contact assay, alkaline phosphatase activity, mineralized matrix deposition analysis by alizarin red staining, as well as evaluation of angiogenic potential and vascular endothelial growth factor release using ST2 cells. Thus, this investigation allows gaining a deeper insight into the biological performance of different inorganic material categories, and to critically compare the different possible solutions, as bone/tissue substitutes for enhanced healing and repair, in terms of bioactivity and regenerative potential.
不同的生物活性玻璃（BGs）、生物陶瓷及其复合材料在生物响应性和血管生成潜力方面得到了广泛的分析。特别是，考虑了几种无机材料，即广泛使用的 45S5BG、结晶倾向低的实验性 BG、另外三种掺杂锶和/或镁的实验性 BG、商业羟基磷灰石（HA）以及两种 BG-HA 复合材料（BG 和 HA 的比例不同）。所有这些材料都是专门制备的，并通过广泛的生物学分析进行体外测试，例如 MC3T3-E1 细胞通过直接接触测定的活力和增殖、碱性磷酸酶活性、茜素红染色分析矿化基质沉积，以及使用 ST2 细胞评估血管生成潜力和血管内皮生长因子释放。因此，这项研究使我们能够更深入地了解不同无机材料类别在生物性能方面的表现，并在增强愈合和修复方面作为骨/组织替代物，从生物活性和再生潜力方面对不同的可能解决方案进行批判性比较。
