Hendry S H, Jones E G
Neuroscience. 1985 Sep;16(1):57-68. doi: 10.1016/0306-4522(85)90047-8.
Immunocytochemical and electron microscopic methods were used to examine neurons in regio superior of rat hippocampus displaying cholecystokinin octapeptide-like immunoreactivity. Cholecystokinin-immunoreactive synaptic terminals and somata are found in all layers of regio superior but are most numerous in stratum pyramidale. The vast majority of terminals form symmetric synaptic contacts onto the somata and proximal dendrites of hippocampal pyramidal cells and onto smaller dendrites which may also arise from pyramidal cells. A very small number of cholecystokinin-immunoreactive terminals form synapses that appear asymmetric and contact dendritic shafts or spines. The somata of some pyramidal cells receive symmetric synapses from cholecystokinin-immunoreactive terminals that are joined by cytoplasmic bridges to form parts of pericellular baskets. These and adjacent pyramidal cell somata are also contacted by terminals that are not immunoreactive for cholecystokinin. No cholecystokinin-positive terminals contacted the initial segments of pyramidal cell axons. Cholecystokinin-immunoreactive cells are found in all layers of regio superior. Their somata receive a few symmetric synapses, most of which are formed by terminals not immunoreactive for cholecystokinin. Their dendrites receive a greater number of both symmetric and asymmetric contacts, some of which are immunoreactive for cholecystokinin. We conclude the following: The localization of cholecystokinin immunoreactivity in synaptic terminals contacting the somata and dendrites of hippocampal pyramidal cells is consistent with the suggestion that cholecystokinin acts as a neurotransmitter at these sites and at sites in other parts of the cerebral cortex. Results from the present and previous studies suggest that cholecystokinin-like immunoreactivity may co-exist with gamma-aminobutyrate in some non-pyramidal neurons of regio superior. Cholecystokinin-immunoreactive terminals arise mainly from non-pyramidal cells intrinsic to the hippocampus, one class of which appears to be a type of basket cell.
采用免疫细胞化学和电子显微镜方法，对大鼠海马体上部显示胆囊收缩素八肽样免疫反应性的神经元进行了检查。胆囊收缩素免疫反应性突触终末和胞体存在于海马体上部的所有层中，但在锥体层中数量最多。绝大多数终末在海马锥体细胞的胞体和近端树突以及可能也由锥体细胞发出的较小树突上形成对称突触联系。极少数胆囊收缩素免疫反应性终末形成不对称突触并与树突干或棘突接触。一些锥体细胞的胞体接受来自胆囊收缩素免疫反应性终末的对称突触，这些终末通过胞质桥相连形成细胞周篮的一部分。这些以及相邻的锥体细胞胞体也被对胆囊收缩素无免疫反应性的终末所接触。没有胆囊收缩素阳性终末与锥体细胞轴突的起始段接触。胆囊收缩素免疫反应性细胞存在于海马体上部的所有层中。它们的胞体接受少数对称突触，其中大多数由对胆囊收缩素无免疫反应性的终末形成。它们的树突接受更多的对称和不对称联系，其中一些对胆囊收缩素具有免疫反应性。我们得出以下结论：胆囊收缩素免疫反应性在与海马锥体细胞胞体和树突接触的突触终末中的定位，与胆囊收缩素在这些部位以及大脑皮质其他部位作为神经递质起作用的观点一致。本研究和先前研究的结果表明，在海马体上部的一些非锥体神经元中，胆囊收缩素样免疫反应性可能与γ-氨基丁酸共存。胆囊收缩素免疫反应性终末主要源自海马体固有的非锥体细胞，其中一类似乎是篮状细胞。
