Sorenson R L, Elde R P, Seybold V
Diabetes. 1979 Oct;28(10):899-904. doi: 10.2337/diab.28.10.899.
The rate of insulin, glucagon, and somatostatin secretion was measured from isolated rat islets maintained in a perifusion system. The effect of norepinephrine (NE) was simultaneously determined on the release rate of all three hormones. Norepinephrine was employed at an acute dose of 10 micrometers and in graded doses from 1 nM to 10 micrometers in the presence of high (22 mM) and low (1.4 mM) glucose conditions, insulin secretion was maximally inhibited at 10 micrometers NE concentration and was significantly depressed at 100 mM NE concentration. Under both high and low glucose conditions, glucagon release was maximally stimulated at 10 micrometers NE concentration and was significantly elevated at 10 nM NE concentration. Under high and low glucose conditions, somatostatin release was inhibited by 10 micrometers NE concentration and was significantly depressed at 100 nM NE concentration. During the initial maximal stimulation of glucagon, NE inhibition of somatostatin and insulin was prevented, possibly by the high level of glucagon released. A paracrine effect of glucagon on beta and delta cells is proposed.
从维持在灌流系统中的分离大鼠胰岛中测量胰岛素、胰高血糖素和生长抑素的分泌速率。同时测定去甲肾上腺素（NE）对这三种激素释放速率的影响。在高（22 mM）和低（1.4 mM）葡萄糖条件下，以10微摩尔的急性剂量和从1纳摩尔到10微摩尔的梯度剂量使用去甲肾上腺素，在NE浓度为10微摩尔时胰岛素分泌受到最大抑制，在NE浓度为100微摩尔时显著降低。在高糖和低糖条件下，胰高血糖素释放在NE浓度为10微摩尔时受到最大刺激，在NE浓度为10纳摩尔时显著升高。在高糖和低糖条件下，生长抑素释放在NE浓度为10微摩尔时受到抑制，在NE浓度为100纳摩尔时显著降低。在胰高血糖素最初的最大刺激期间，NE对生长抑素和胰岛素的抑制作用被阻止，这可能是由于释放的胰高血糖素水平较高。提出了胰高血糖素对β细胞和δ细胞的旁分泌作用。
