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[用显色底物测定血小板的抗纤溶酶活性]

[Determination of the antiplasmin activity of platelets with a chromogenic substrate].

作者信息

Campo G, Ciaffoni S

出版信息

Quad Sclavo Diagn. 1985 Sep;21(3):360-9.

PMID:3835576
Abstract

The synthesis of chromogenic substrate has opened new prospects to the study of haemostasis factors and particularly to the determination of coagulation inhibitors and components of fibrinolysis. In this paper we propose a new and simple method to anti-plasmin activity of platelets, by using H-D-Valyl-L-Leucyl-L-Lysine-p-nitroanilide HCl (S-2251) (Kabi Company, Sweden) as a chromogenic substrate.

摘要

发色底物的合成,为止血因子的研究,尤其是凝血抑制剂和纤维蛋白溶解成分的测定开辟了新前景。在本文中,我们提出了一种新的、简单的方法来检测血小板的抗纤溶酶活性,该方法使用H-D-缬氨酰-L-亮氨酰-L-赖氨酸对硝基苯胺盐酸盐(S-2251)(瑞典卡比公司)作为发色底物。

相似文献

1
[Determination of the antiplasmin activity of platelets with a chromogenic substrate].[用显色底物测定血小板的抗纤溶酶活性]
Quad Sclavo Diagn. 1985 Sep;21(3):360-9.
2
[Determination of kallikrein in gel-filtered urine using the chromogenic substrate D-valyl-L-leucyl-L-arginyl-4-nitroanilide].
Z Med Lab Diagn. 1991;32(2):116-7.
3
Assessment of inhibitors with chromogenic substrates.用显色底物评估抑制剂。
J Clin Pathol Suppl (R Coll Pathol). 1980;14:48-9.
4
Modification of the immediate plasmin inhibition assay to secure linearity of the reference curve with the chromogenic substrate S-2251.对即时纤溶酶抑制试验进行改进,以确保与发色底物S-2251的参考曲线呈线性关系。
Thromb Haemost. 1984 Apr 30;51(2):298.
5
Use of chromogenic substrate S-2251 for determination of plasminogen activator in rat ovaries.
Thromb Haemost. 1981 Aug 28;46(2):507-10.
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Kinetic peculiarities of human tissue kallikrein: 1--substrate activation in the catalyzed hydrolysis of H-D-valyl-L-leucyl-L-arginine 4-nitroanilide and H-D-valyl-L-leucyl-L-lysine 4-nitroanilide; 2--substrate inhibition in the catalyzed hydrolysis of N alpha-p-tosyl-L-arginine methyl ester.人组织激肽释放酶的动力学特性:1. 在催化水解H-D-缬氨酰-L-亮氨酰-L-精氨酸4-硝基苯胺和H-D-缬氨酰-L-亮氨酰-L-赖氨酸4-硝基苯胺过程中的底物激活;2. 在催化水解Nα-对甲苯磺酰-L-精氨酸甲酯过程中的底物抑制。
Arch Biochem Biophys. 2002 Apr 1;400(1):7-14. doi: 10.1006/abbi.2002.2764.
7
[Use of chromogenic substrates in the clarification of disorders in the early stages of blood coagulation].[显色底物在凝血早期疾病诊断中的应用]
Folia Haematol Int Mag Klin Morphol Blutforsch. 1982;109(1):115-20.
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[Diagnosis of increased thrombotic tendency with chromogenic substrates].[用发色底物诊断血栓形成倾向增加]
Folia Haematol Int Mag Klin Morphol Blutforsch. 1982;109(1):135-41.
9
[Clinical evaluations of antithrombin III and alpha 2-plasmin inhibitor assay method with chromogenic substrates (S-2238, S-2251 (author's transl)].抗凝血酶III及α2-纤溶酶抑制剂显色底物法(S-2238、S-2251)的临床评价(作者译)
Rinsho Byori. 1981 Feb;29(2):179-82.
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Functional activities and concentrations of plasmin inhibitors in normal subjects and D.I.C. patients.正常受试者和弥散性血管内凝血患者纤溶酶抑制剂的功能活性及浓度
Thromb Haemost. 1982 Jun 28;47(3):275-7.