Ciftci Hakan, Tas Nilay, Cebeci Zubeyir, Kokturk Sibel, Cirrik Selma, Noyan Tevfik
Department of Anesthesiology and Reanimation, Taskopru State Hospital, Kastamonu, Turkiye.
Department of Anesthesiology and Reanimation, Ordu University Faculty of Medicine, Ordu, Turkiye.
North Clin Istanb. 2024 Jan 29;11(1):1-9. doi: 10.14744/nci.2023.07888. eCollection 2024.
Cerebral ischemia-reperfusion (I/R) injury causes neurological dysfunction and cell death. Sugammadex, as a large molecule, is normally difficult to pass through the blood-brain barrier (BBB). In ischemia, molecules can pass into the brain tissue. In this study, we aimed to evaluate the effect of sugammadex in the presence of cerebral I/R damage in rats with a general anesthesia model with sevoflurane and rocuronium.
Rats were divided into 7 groups; Group 1 (Control), Group 2 (Sham), Group 3 (Sevoflurane), Group 4 (Sugammadex), Group 5 (Sevoflurane + Rocuronium), Group 6 (Sevoflurane + Sugammadex), Group 7 (Sevoflurane + Rocuronium + Sugammadex). Brain tissues of rats with cerebral I/R damage with bilateral carotid occlusion were removed. Tissue Malondialdehyde (MDA), Myeloperoxidase (MPO), and Superoxide dismutase (SOD) levels were examined with ELISA and apoptosis was examined by Caspase-3.
The number of caspase-3 positive cells decreased the most in Group 4 compared to the other groups. Group 4's mean MDA and MPO levels were lower than Group 2. There was no significant difference in terms of SOD levels.
The apoptotic effect of sugammadex was lowest compared to other agent groups, and it did not increase oxidative damage as much as the other groups.
脑缺血再灌注（I/R）损伤会导致神经功能障碍和细胞死亡。舒更葡糖作为一种大分子物质，通常难以通过血脑屏障（BBB）。在缺血状态下，分子可以进入脑组织。在本研究中，我们旨在评估在七氟醚和罗库溴铵全身麻醉模型的大鼠脑I/R损伤情况下舒更葡糖的作用。
将大鼠分为7组；第1组（对照组）、第2组（假手术组）、第3组（七氟醚组）、第4组（舒更葡糖组）、第5组（七氟醚+罗库溴铵组）、第6组（七氟醚+舒更葡糖组）、第7组（七氟醚+罗库溴铵+舒更葡糖组）。对双侧颈动脉闭塞造成脑I/R损伤的大鼠脑组织进行取材。采用酶联免疫吸附测定法检测组织丙二醛（MDA）、髓过氧化物酶（MPO）和超氧化物歧化酶（SOD）水平，通过Caspase-3检测细胞凋亡情况。
与其他组相比，第4组中Caspase-3阳性细胞数量减少最多。第4组的平均MDA和MPO水平低于第2组。SOD水平方面无显著差异。
与其他药物组相比，舒更葡糖的凋亡作用最低，且其造成的氧化损伤也不如其他组严重。
