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Restriction map, partial cloning and localization of 9S and 12S kinetoplast RNA genes on the maxicircle component of the kinetoplast DNA of Leishmania tarentolae.

作者信息

Masuda H, Simpson L, Rosenblatt H, Simpson A M

出版信息

Gene. 1979 May;6(1):51-73. doi: 10.1016/0378-1119(79)90085-4.

DOI:10.1016/0378-1119(79)90085-4
PMID:383578
Abstract

We have constructed a restriction map of the maxicircle component of the kinetoplast DNA of Leishmania tarentolae for the enzymes EcoRI, Bam HI, HaeIII, HpaII, SalI, BglII and HindIII. The 9 and 12S kinetoplast RNAs were localized on this map. Two fragments of this maxicircle molecule were cloned in the bacterial plasmid, pBR322, including a 4.4 . 10(6) dalton EcoRI/BamHI fragment which contains the 9 and 12S RNA genes.

摘要

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