Int J Oral Maxillofac Implants. 2024 Aug 29;39(4):603-614. doi: 10.11607/jomi.10745.
To examine the behavior and function of human gingival fibroblasts growing on healing abutments with or without laser-textured topography.
Human primary gingival connective tissue fibroblasts were cultured on healing abutments with machined or laser-textured (Laser-Lok, BioHorizons) surfaces. Cellular and molecular responses were evaluated by a variety of tests, including cell density assay (WST-1), fluorescence microscopy, real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR), and detachment tests.
The machined surface showed monodirectional traces and scratches from milling, whereas the laser-textured surface showed a distinct morphology consisting of monodirectional mesoscale channels (15-μm pitch) and woven oblique microridges formed within the channels. There were no differences in initial fibroblast attachment, subsequent fibroblast proliferation, or collagen production between the machined and laser-textured surfaces. Fibroblasts growing on a laser-textured surface were found to spread in one direction along the mesochannels, while cells growing on machined surfaces tended to spread randomly. Fibroblasts on laser-textured surfaces were 1.8 times more resistant to detachment than those on machined surfaces. An adhesive glycoprotein (fibronectin) and transmembrane adhesion linker gene (integrin β-1) were upregulated on laser-textured surfaces.
The increased fibroblast retention, uniform growth, and increased transcription of cell adhesion proteins compellingly explain the enhanced tissue-level response to laser-created and hybrid-textured titanium surfaces. These results provide a cellular and molecular rationale for the tissue reaction to this unique surface; in addition, they support its extended use, from implants and healing abutments to diverse prosthetic components where enhanced soft tissue responses would be desirable.
研究人牙龈成纤维细胞在具有和不具有激光纹理形貌的愈合基台上的生长行为和功能。
将人原代牙龈连接组织成纤维细胞培养在机械加工或激光纹理（Laser-Lok，BioHorizons）表面的愈合基台上。通过多种试验评估细胞和分子反应，包括细胞密度测定（WST-1）、荧光显微镜、实时定量逆转录聚合酶链反应（qRT-PCR）和脱落试验。
机械加工表面显示出单向痕迹和铣削划痕，而激光纹理表面则呈现出明显的形貌，由单向中尺度通道（15μm 节距）和在通道内形成的编织斜微脊组成。在初始成纤维细胞附着、随后的成纤维细胞增殖或胶原产生方面，机械加工表面和激光纹理表面之间没有差异。在激光纹理表面上，发现成纤维细胞沿着中尺度通道单向扩散，而在机械加工表面上生长的细胞则倾向于随机扩散。在激光纹理表面上的成纤维细胞比在机械加工表面上的成纤维细胞更能抵抗脱落。在激光纹理表面上，一种黏附糖蛋白（纤连蛋白）和跨膜黏附连接子基因（整合素β-1）的表达上调。
成纤维细胞保留增加、均匀生长和细胞黏附蛋白转录增加，有力地解释了对激光创建和混合纹理钛表面的增强组织水平反应。这些结果为这种独特表面的组织反应提供了细胞和分子基础；此外，它们支持其广泛应用，从植入物和愈合基台到各种需要增强软组织反应的修复组件。
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